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Product Details
MA5-46621
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
204
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Bcl-2.
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.03% Proclin 300
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937693
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the N-terminus of the Human BCL2/Bcl-2, and conjugated with APC under optimum conditions, the unreacted APC was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl 2; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50
Gene Aliases: Bcl-2; BCL2; PPP1R50
UniProt ID: (Human) P10415
Entrez Gene ID: (Human) 596
protease binding
protein binding
transcription factor binding
channel activity
channel inhibitor activity
ubiquitin protein ligase binding
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
protein heterodimerization activity
BH3 domain binding
protein phosphatase 2A binding
protein polyubiquitination
ossification
ovarian follicle development
metanephros development
branching involved in ureteric bud morphogenesis
behavioral fear response
B cell homeostasis
release of cytochrome c from mitochondria
regulation of cell-matrix adhesion
lymphoid progenitor cell differentiation
B cell lineage commitment
response to ischemia
renal system process
protein dephosphorylation
melanin metabolic process
regulation of nitrogen utilization
apoptotic process
humoral immune response
cellular response to DNA damage stimulus
actin filament organization
axonogenesis
female pregnancy
cell aging
male gonad development
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
intrinsic apoptotic signaling pathway in response to oxidative stress
response to radiation
response to toxic substance
post-embryonic development
response to iron ion
response to UV-B
response to gamma radiation
regulation of gene expression
negative regulation of autophagy
negative regulation of calcium ion transport into cytosol
regulation of glycoprotein biosynthetic process
mesenchymal cell development
positive regulation of neuron maturation
positive regulation of smooth muscle cell migration
cell growth
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
cochlear nucleus development
gland morphogenesis
regulation of transmembrane transporter activity
negative regulation of ossification
positive regulation of cell growth
negative regulation of cell growth
melanocyte differentiation
negative regulation of cell migration
positive regulation of B cell proliferation
hair follicle morphogenesis
axon regeneration
regulation of protein stability
endoplasmic reticulum calcium ion homeostasis
glomerulus development
negative regulation of cellular pH reduction
negative regulation of myeloid cell apoptotic process
T cell differentiation in thymus
positive regulation of peptidyl-serine phosphorylation
negative regulation of osteoblast proliferation
response to cytokine
response to nicotine
organ growth
positive regulation of multicellular organism growth
B cell proliferation
cellular response to glucose starvation
response to drug
response to hydrogen peroxide
T cell homeostasis
negative regulation of apoptotic process
positive regulation of catalytic activity
CD8-positive, alpha-beta T cell lineage commitment
regulation of protein homodimerization activity
regulation of protein heterodimerization activity
negative regulation of neuron apoptotic process
ear development
regulation of viral genome replication
positive regulation of melanocyte differentiation
negative regulation of retinal cell programmed cell death
regulation of mitochondrial membrane permeability
focal adhesion assembly
spleen development
thymus development
digestive tract morphogenesis
oocyte development
positive regulation of skeletal muscle fiber development
pigment granule organization
homeostasis of number of cells within a tissue
B cell receptor signaling pathway
response to glucocorticoid
neuron apoptotic process
defense response to virus
regulation of mitochondrial membrane potential
negative regulation of mitochondrial depolarization
regulation of calcium ion transport
transmembrane transport
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to organic substance
cellular response to hypoxia
reactive oxygen species metabolic process
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
negative regulation of G1/S transition of mitotic cell cycle
negative regulation of reactive oxygen species metabolic process
negative regulation of anoikis
negative regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
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