Proteintech

MAD2L1 Polyclonal Antibody

MAD2L1 Antibody in Immunocytochemistry (ICC/IF)

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MAD2L1 Antibody (10337-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MAD2L1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MAD2L1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10337-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MAD2L1 Fusion Protein Ag0307 (16-185 aa encoded by BC000356)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SAEIVAEFF SFGINSILYQ RGIYPSETFT RVQKYGLTLL VTTDLELIKY LNNVVEQLKD WLYKCSVQKL VVVISNIESG EVLERWQFDI ECDKTAKDDS APREKSQKAI QDEIRSVIRQ ITATVTFLPL LEVSCSFDLL IYTDKDLVVP EKWEESGPQF ITNSEEVRLR S (16-185 aa encoded by BC000356)

Target Information

Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3.

Thoracic cancer

Zhou D,Liu T,Rao X,Jie X,Chen Y,Wu Z,Deng H,Zhang D,Wang J,Wu G

Mon May 01 00:00:00 EDT 2023

Mitogen-induced defective mitosis transforms neural progenitor cells.

Neuro-oncology

Omairi HK,Grisdale CJ,Meode M,Bohm AK,Black S,Adam NJ,Chapman CP,Maroilley T,Kelly JJ,Tarailo-Graovac M,Jones SJM,Blough MD,Cairncross JG

Tue Oct 03 00:00:00 EDT 2023

Nivalenol affects Cyclin B1 level and activates SAC for cell cycle progression in mouse oocyte meiosis.

Cell proliferation

Lu PS,Wang Y,Jiao L,Sun SC

Thu Sep 01 00:00:00 EDT 2022

Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation.

Oxidative medicine and cellular longevity

Cao M,Ma R,Li H,Cui J,Zhang C,Zhao J

Mon Apr 04 00:00:00 EDT 2022

Septin 4 controls CCNB1 stabilization via APC/C^CDC20 during meiotic G2/M transition in mouse oocytes.

Journal of cellular physiology

Chen L,Ouyang YC,Li J,Qiao JY,Gu LJ,Wang ZB,Hou Y,Schatten H,Sun QY

Sat Jan 01 00:00:00 EST 2022

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

International journal of molecular sciences

Morani F,Bisceglia L,Rosini G,Mutti L,Melaiu O,Landi S,Gemignani F

Mon Mar 08 00:00:00 EST 2021

Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.

The Journal of cell biology

Wu T,Lane SIR,Morgan SL,Tang F,Jones KT

Mon Oct 04 00:00:00 EDT 2021

Melatonin promotes in vitro maturation of vitrified-warmed mouse GV oocytes potentially by modulating MAD2 protein expression of SAC component through MTRs.

Cryobiology

Yang J,Guo S,Pan B,Qazi IH,Qin J,Zang S,Han H,Meng Q,Zhou G

Fri Oct 01 00:00:00 EDT 2021

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

Science advances

Zhou C,Miao Y,Cui Z,ShiYang X,Zhang Y,Xiong B

Wed Apr 01 00:00:00 EDT 2020

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Nucleic acids research

Yuan F,Jin X,Li D,Song Y,Zhang N,Yang X,Wang L,Zhu WG,Tian C,Zhao Y

Thu Sep 05 00:00:00 EDT 2019

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Nucleic acids research

Yuan F,Jin X,Li D,Song Y,Zhang N,Yang X,Wang L,Zhu WG,Tian C,Zhao Y

Thu Sep 05 00:00:00 EDT 2019

miR-30a-3p Targets MAD2L1 and Regulates Proliferation of Gastric Cancer Cells.

OncoTargets and therapy

Wang Y,Wang F,He J,Du J,Zhang H,Shi H,Chen Y,Wei Y,Xue W,Yan J,Feng Y,Gao Y,Li D,Han J,Zhang J

Tue Apr 12 00:00:00 EDT 2022

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Oncogene

Zhu ZC,Liu JW,Li K,Zheng J,Xiong ZQ

Tue May 01 00:00:00 EDT 2018

KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.

Oncogene

Zhu ZC,Liu JW,Li K,Zheng J,Xiong ZQ

Tue May 01 00:00:00 EDT 2018

Nucleoporin35 is a novel microtubule associated protein functioning in oocyte meiotic spindle architecture.

Experimental cell research

Chen F,Jiao XF,Zhang JY,Wu D,Ding ZM,Wang YS,Miao YL,Huo LJ

Mon Oct 15 00:00:00 EDT 2018

Nucleoporin35 is a novel microtubule associated protein functioning in oocyte meiotic spindle architecture.

Experimental cell research

Chen F,Jiao XF,Zhang JY,Wu D,Ding ZM,Wang YS,Miao YL,Huo LJ

Mon Oct 15 00:00:00 EDT 2018

MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.

Cancer research

Liu X,Zhou Y,Liu X,Peng A,Gong H,Huang L,Ji K,Petersen RB,Zheng L,Huang K

Sat Nov 15 00:00:00 EST 2014

MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.

Cancer research

Liu X,Zhou Y,Liu X,Peng A,Gong H,Huang L,Ji K,Petersen RB,Zheng L,Huang K

Sat Nov 15 00:00:00 EST 2014

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Wu J,Chen W,Yin Y,Zheng Z,Zou G

Sun Jun 01 00:00:00 EDT 2014

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Wu J,Chen W,Yin Y,Zheng Z,Zou G

Sun Jun 01 00:00:00 EDT 2014

Expression of the mitotic-arrest deficiency 2 is associated with chemotherapy resistance in ovarian serous adenocarcinoma.

Oncology reports

Nakano Y,Sumi T,Teramae M,Morishita M,Fukuda T,Terada H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Mon Oct 01 00:00:00 EDT 2012

Expression of the mitotic-arrest deficiency 2 is associated with chemotherapy resistance in ovarian serous adenocarcinoma.

Oncology reports

Nakano Y,Sumi T,Teramae M,Morishita M,Fukuda T,Terada H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Mon Oct 01 00:00:00 EDT 2012

Mitotic arrest deficiency 2 induces carcinogenesis in mucinous ovarian tumors.

Oncology letters

Nakano Y,Sumi T,Morishita M,Fukuda T,Nobeyama H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Wed Feb 01 00:00:00 EST 2012

Mitotic arrest deficiency 2 induces carcinogenesis in mucinous ovarian tumors.

Oncology letters

Nakano Y,Sumi T,Morishita M,Fukuda T,Nobeyama H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Wed Feb 01 00:00:00 EST 2012

Expression of mitotic-arrest deficiency 2 predicts the efficacy of neoadjuvant chemotherapy for locally advanced uterine cervical cancer.

Experimental and therapeutic medicine

Morishita M,Sumi T,Nakano Y,Teramae M,Fukuda T,Nobeyama H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Wed Feb 01 00:00:00 EST 2012

Expression of mitotic-arrest deficiency 2 predicts the efficacy of neoadjuvant chemotherapy for locally advanced uterine cervical cancer.

Experimental and therapeutic medicine

Morishita M,Sumi T,Nakano Y,Teramae M,Fukuda T,Nobeyama H,Yoshida H,Matsumoto Y,Yasui T,Ishiko O

Wed Feb 01 00:00:00 EST 2012

Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcoma.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Yu L,Guo WC,Zhao SH,Tang J,Chen JL

Mon Mar 01 00:00:00 EST 2010

Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcoma.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Yu L,Guo WC,Zhao SH,Tang J,Chen JL

Mon Mar 01 00:00:00 EST 2010

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Cancer research

He F,Mo L,Zheng XY,Hu C,Lepor H,Lee EY,Sun TT,Wu XR

Tue Dec 15 00:00:00 EST 2009

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Cancer research

He F,Mo L,Zheng XY,Hu C,Lepor H,Lee EY,Sun TT,Wu XR

Tue Dec 15 00:00:00 EST 2009

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Cancer research

He F,Mo L,Zheng XY,Hu C,Lepor H,Lee EY,Sun TT,Wu XR

Tue Dec 15 00:00:00 EST 2009

Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.

Cancer research

He F,Mo L,Zheng XY,Hu C,Lepor H,Lee EY,Sun TT,Wu XR

Tue Dec 15 00:00:00 EST 2009

Bioinformatics

Protein Aliases: HsMAD2; MAD2 (mitotic arrest deficient, homolog)-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; MadL1; Mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; Mitotic spindle assembly checkpoint protein MAD2A

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Gene Aliases: AA673185; HSMAD2; MAD2; Mad2a; MAD2L1

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UniProt ID: (Human) Q13257, (Mouse) Q9Z1B5

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Entrez Gene ID: (Human) 4085, (Rat) 297176, (Mouse) 56150

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Function(s)

protein binding identical protein binding protein homodimerization activity

Process(es)

mitotic sister chromatid segregation mitotic cell cycle checkpoint mitotic spindle assembly checkpoint negative regulation of protein catabolic process negative regulation of apoptotic process negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle spindle assembly checkpoint negative regulation of ubiquitin protein ligase activity cell cycle mitotic nuclear division negative regulation of mitotic metaphase/anaphase transition cell division sister chromatid cohesion anaphase-promoting complex-dependent catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MAD2L1 Polyclonal Antibody

MAD2L1 Antibody (10337-1-AP) in ICC/IF

Product Details

10337-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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