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FIGURE: 1 / 1
MAFB Antibody (PA5-87620) in WB
Western blot analysis of MAFB in extracts of various cell lines. Samples were incubated with MAFB Polyclonal antibody (Product # PA5-87620) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-87620
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 184-323 of human MAFB (NP_0054522)
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804295
Product Specific Information
Immunogen sequence: PTSHPGPGPH ATASATAAGG NGSVEDRFSD DQLVSMSVRE LNRHLRGFTK DEVIRLKQKR RTLKNRGYAQ SCRYKRVQQK HHLENEKTQL IQQVEQLKQE VSRLARERDA YKVKCEKLAN SGFREAGSTS DSPSSPEFFL; Positive Samples: Mouse liver, Mouse kidney, Rat liver; Cellular Location: Nucleus
Target Information
The protein encoded by this gene is a basic leucine zipper transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Kreisler; Kreisler (maf-related) leucine zipper protein; Kreisler maf-related leucine zipper homolog (b-maf); Maf-B; MGC43127; Segmentation protein Kr; Transcription factor Maf-1; Transcription factor MafB; v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
Gene Aliases: b-maf; kr; Kreisler; Krml; Krml1; Maf1; Mafb
UniProt ID: (Rat) P54842, (Mouse) P54841
Entrez Gene ID: (Rat) 54264, (Mouse) 16658
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
sequence-specific DNA binding
basic leucine zipper transcription factor
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
segment specification
respiratory gaseous exchange
rhombomere 5 development
rhombomere 6 development
T cell differentiation in thymus
brain segmentation
inner ear morphogenesis
negative regulation of erythrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
thymus development
regulation of transcription, DNA-templated
negative regulation of osteoclast differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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