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FIGURE: 1 / 1
MAGP-2 Antibody (600-401-937) in WB
Western blot using Rocklands affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4° C. Personal Communication, Michael Birrer, CCR-NCI, Bethesda, MD
Product Details
600-401-937
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human MAGP-2 protein.
Conjugate
Form
Liquid
Concentration
0.95 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human MAGP-2 protein. A BLAST analysis was used to suggest cross reactivity with MAGP-2 protein from human and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with MAGP-2 from mouse, dog and rat sources based on partial (~75%) sequence homology. Reactivity against homologues from other sources is not known.
Target Information
This gene encodes a 25-kDa microfibril-associated glycoprotein which is rich in serine and threonine residues. It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31- kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAGP-2; MFAP-5; Microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2; Microfibrillar-associated protein 5; MP25; THE1A-MFAP5
Gene Aliases: AAT9; MAGP-2; MAGP2; MFAP-5; MFAP5; MP25
UniProt ID: (Human) Q13361
Entrez Gene ID: (Human) 8076
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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