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MAP1LC3C Antibody (PA5-100600) in ICC/IF
Immunocytochemistry analysis of MAP1LC3C in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MAP1LC3C polyclonal antibody (Product # PA5-100600) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100600
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAP1LC3C(Accession Q9BXW4), corresponding to amino acid residues D110-L147.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850107
Product Specific Information
Antibody detects endogenous levels of total LC3C.
Target Information
MAP1LC3C, also named as LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Autoph; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; LC3-like protein 2; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; MAP1A/MAP1B light chain 3 C; Microtubule-associated protein 1 light chain 3 gamma; Microtubule-associated proteins 1A/1B light chain 3C
Gene Aliases: ATG8J; LC3C; MAP1LC3C
UniProt ID: (Human) Q9BXW4
Entrez Gene ID: (Human) 440738
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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