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MAP2 Antibody (OSM00036G-500UG) in IHC (P)
Immunohistochemistry analysis of paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Osenses rabbit anti Gp combined with HRP polymer from Leica following m... View More
Product Details
OSM00036G-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 350-400 of mouse MAP2 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in rat.
Specificity of this antibody: MAP-2.
Target Information
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AC091465.1; DKFZp686I2148; MAP-2; map-2a; map2ab; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143
Gene Aliases: G1-397-34; MAP-2; Map2; MAP2R; Mtap-2; Mtap2; repro4
UniProt ID: (Mouse) P20357, (Rat) P15146
Entrez Gene ID: (Mouse) 17756, (Rat) 25595
Performance Guarantee
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