Invitrogen

MAP2 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

10 Published Figures

21 References

View all (71) MAP2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MAP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

MAP2 Antibody (PA1-10005) in ICC/IF

Immunofluorescent analysis of MAP2 in cortical neuron-glial cell culture. The culture was prepared from an E20 rat and stained using a MAP2 polyclonal antibody (Product # PA1-10005) at a dilution of 1:10,000 as seen in red, and a MAP-tau monoclonal antibody at a dilution of 1:2,000 as seen in green, and with DAPI staining the nuclear DNA in blue. The MAP2 antibody stains dendrites and perikarya of neurons, while the antibody to MAP-tau labels neuronal perikarya, dendrites and also axonal processes. As a result perikarya and dendrites appea... View More

MAP2 Antibody in Western Blot, Immunohistochemistry (Frozen) (WB, IHC (F))

MAP2 Antibody in Immunohistochemistry (IHC)

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MAP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of MAP2 was observed in Mouse Brain in comparison to Mouse Heart and Mouse Skeletal Muscle using Anti-MAP2 Polyclonal Antibody (Product # PA1-10005) in Western Blot. {RE}

Product Details

PA1-10005

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000-1:50,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:5,000-1:10,000

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:5,000-1:10,000

View 11 publications 11 publications

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076848

Product Specific Information

PA1-10005 was raised against recombinant constructs of the entire human projection domain, and so recognizes only the high molecular MAP2 forms, MAP2A and MAP2B.| For long term storage, store at 4°C.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuronal Differentiation and Outgrowth Effect of Thymol in Trachyspermum ammi Seed Extract via BDNF/TrkB Signaling Pathway in Prenatal Maternal Supplementation and Primary Hippocampal Culture.

International journal of molecular sciences

Timalsina B,Haque MN,Dash R,Choi HJ,Ghimire N,Moon IS

Published figure using MAP2 polyclonal antibody (Product # PA1-10005) in Western Blot

Wed May 10 00:00:00 EDT 2023

Neuronal Differentiation and Outgrowth Effect of Thymol in Trachyspermum ammi Seed Extract via BDNF/TrkB Signaling Pathway in Prenatal Maternal Supplementation and Primary Hippocampal Culture.

International journal of molecular sciences

Timalsina B,Haque MN,Dash R,Choi HJ,Ghimire N,Moon IS

Published figure using MAP2 polyclonal antibody (Product # PA1-10005) in Western Blot

Wed May 10 00:00:00 EDT 2023

Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal Activity.

Biomedicines

Scalise S,Zannino C,Lucchino V,Lo Conte M,Scaramuzzino L,Cifelli P,D'Andrea T,Martinello K,Fucile S,Palma E,Gambardella A,Ruffolo G,Cuda G,Parrotta EI

Published figure using MAP2 polyclonal antibody (Product # PA1-10005) in Immunocytochemistry

Thu May 05 00:00:00 EDT 2022

The Role of Nuclear Factor-Kappa B in Fibrinogen-Induced Inflammatory Responses in Cultured Primary Neurons.

Biomolecules

Sulimai N,Brown J,Lominadze D

PA1-10005 was used in Immunohistochemistry-immunofluorescence to suggest that TBI-induced neurodegeneration can involve the direct interaction of extravasated Fg with neurons, resulting in the overexpression of pro-inflammatory cytokines through the activation of transcription factor NF-κB. This may be a mechanism involved in vascular cognitive impairment during neuroinflammatory diseases.

Wed Nov 23 00:00:00 EST 2022

Chemical phenotypes of intrinsic cardiac neurons in the newborn pig (Sus scrofa domesticus Erxleben, 1777).

Journal of morphology

Ragauskas T,Rysevaite-Kyguoliene K,Pauziene N,Inokaitis H,Pauza DH

PA1-10005 was used in Immunohistochemistry to demonstrate that intrinsic cardiac ganglionated plexus is not fully developed in the newborn pig despite of dense network of neuronal processes and numerous signs of neural contacts within ganglia.

Sat Jan 01 00:00:00 EST 2022

A Learning Based Framework for Disease Prediction from Images of Human-Derived Pluripotent Stem Cells of Schizophrenia Patients.

Neuroinformatics

Fularczyk N,Di Re J,Stertz L,Walss-Bass C,Laezza F,Labate D

PA1-10005 was used in Immunocytochemistry to investigate structural alterations in the neuronal cytoskeleton of hiPSCs of schizophrenia (SCZ) patients.

Fri Apr 01 00:00:00 EDT 2022

A Three-Dimensional Brain-on-a-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.

Methods in molecular biology (Clifton, N.J.)

Liu L,Koo Y,Russell T,Yun Y

PA1-10005 was used in Immunocytochemistry-immunofluorescence to describe a method to construct a brain-on-a-chip with human induced pluripotent stem cell (iPSC)-derived neurons and astrocytes to simulate human brain behavior.

Fri Jun 24 00:00:00 EDT 2022

Autism Spectrum Disorder Risk Factor Met Regulates the Organization of Inhibitory Synapses.

Frontiers in molecular neuroscience

Jeckel P,Kriebel M,Volkmer H

PA1-10005 was used in Immunohistochemistry to provide evidence for Met-dependent gephyrin clustering via activation of mTOR.

Tue Jun 01 00:00:00 EDT 2021

Autism Spectrum Disorder Risk Factor Met Regulates the Organization of Inhibitory Synapses.

Frontiers in molecular neuroscience

Jeckel P,Kriebel M,Volkmer H

PA1-10005 was used in Immunohistochemistry to provide evidence for Met-dependent gephyrin clustering via activation of mTOR.

Tue Jun 01 00:00:00 EDT 2021

Autism Spectrum Disorder Risk Factor Met Regulates the Organization of Inhibitory Synapses.

Frontiers in molecular neuroscience

Jeckel P,Kriebel M,Volkmer H

PA1-10005 was used in Immunohistochemistry to provide evidence for Met-dependent gephyrin clustering via activation of mTOR.

Tue Jun 01 00:00:00 EDT 2021

The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease.

eLife

de Rus Jacquet A,Tancredi JL,Lemire AL,DeSantis MC,Li WP,O'Shea EK

Published figure using MAP2 polyclonal antibody (Product # PA1-10005) in Immunocytochemistry

Thu Sep 30 00:00:00 EDT 2021

Precise Ultrasound Neuromodulation in a Deep Brain Region Using Nano Gas Vesicles as Actuators.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Hou X,Qiu Z,Xian Q,Kala S,Jing J,Wong KF,Zhu J,Guo J,Zhu T,Yang M,Sun L

Published figure using MAP2 polyclonal antibody (Product # PA1-10005) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Delivering progranulin to neuronal lysosomes protects against excitotoxicity.

The Journal of biological chemistry

Davis SE,Roth JR,Aljabi Q,Hakim AR,Savell KE,Day JJ,Arrant AE

PA1-10005 was used in Western Blot to show that progranulin's protection against excitotoxicity does not require extracellular progranulin, but is mediated through lysosomes, providing a mechanistic link between progranulin's lysosomal and neurotrophic effects.

Wed Sep 01 00:00:00 EDT 2021

Delivering progranulin to neuronal lysosomes protects against excitotoxicity.

The Journal of biological chemistry

Davis SE,Roth JR,Aljabi Q,Hakim AR,Savell KE,Day JJ,Arrant AE

Wed Sep 01 00:00:00 EDT 2021

A peptide inhibitor of Tau-SH3 interactions ameliorates amyloid-β toxicity.

Neurobiology of disease

Rush T,Roth JR,Thompson SJ,Aldaher AR,Cochran JN,Roberson ED

PA1-10005 was used in Immunocytochemistry to determine if inhibiting Tau-SH3 interactions ameliorates Aβ toxicity.

Sat Feb 01 00:00:00 EST 2020

Targeted Neurostimulation in Mouse Brains with Non-invasive Ultrasound.

Cell reports

Qiu Z,Kala S,Guo J,Xian Q,Zhu J,Zhu T,Hou X,Wong KF,Yang M,Wang H,Sun L

PA1-10005 was used in Immunocytochemistry to demonstrate a neuron-specific and region-targeted neural excitation strategy using non-invasive ultrasound through activation of heterologously expressed mechanosensitive ion channels (MscL-G22S).

Tue Aug 18 00:00:00 EDT 2020

Tau interacts with SHP2 in neuronal systems and in Alzheimer's disease brains.

Journal of cell science

Kim Y,Liu G,Leugers CJ,Mueller JD,Francis MB,Hefti MM,Schneider JA,Lee G

PA1-10005 was used in Proximity Ligation Assay to show a relationship between Tau and protein tyrosine phosphatase SHP2 that can act in nerve growth factor-stimulated neuronal development and in Alzheimer's disease.

Mon Jul 15 00:00:00 EDT 2019

Loss of tau and Fyn reduces compensatory effects of MAP2 for tau and reveals a Fyn-independent effect of tau on calcium.

Journal of neuroscience research

Liu G,Thangavel R,Rysted J,Kim Y,Francis MB,Adams E,Lin Z,Taugher RJ,Wemmie JA,Usachev YM,Lee G

PA1-10005 was used in Western Blotting to hypothesize that in the tau KO, the absence of tau would be compensated by MAP2, especially in the dendrites, where tau-Fyn complexes are of critical importance.

Fri Nov 01 00:00:00 EDT 2019

Imaging of the Axon Initial Segment.

Current protocols in neuroscience

Di Re J,Kayasandik C,Botello-Lins G,Labate D,Laezza F

PA1-10005 was used in Immunohistochemistry, Immunocytochemistry to describe methods for immunolabeling AIS proteins in cultured neurons and brain slices as well as methods for quantifying protein expression and pattern distribution using fluorescent labeling of these proteins.

Sun Sep 01 00:00:00 EDT 2019

Imaging of the Axon Initial Segment.

Current protocols in neuroscience

Di Re J,Kayasandik C,Botello-Lins G,Labate D,Laezza F

Sun Sep 01 00:00:00 EDT 2019

Three-dimensional (3D) tetra-culture brain on chip platform for organophosphate toxicity screening.

Scientific reports

Koo Y,Hawkins BT,Yun Y

PA1-10005 was used in Immunocytochemistry-immunoflourescence to report on a microfluidic-based three-dimensional, four-cell tissue construct consisting of 1) a blood-brain barrier that has dynamic flow and membrane-free culture of the endothelial layer, and 2) an extracellular matrix (ECM)-embedded tissue construct with neuroblastoma, microglia, and astrocytes.

Mon Feb 12 00:00:00 EST 2018

Zika Virus Infects Intermediate Progenitor Cells and Post-mitotic Committed Neurons in Human Fetal Brain Tissues.

Scientific reports

Lin MY,Wang YL,Wu WL,Wolseley V,Tsai MT,Radic V,Thornton ME,Grubbs BH,Chow RH,Huang IC

Published figure using MAP2 polyclonal antibody (Product # PA1-10005) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Genetic and morphological features of human iPSC-derived neurons with chromosome 15q11.2 (BP1-BP2) deletions.

Molecular neuropsychiatry

Das DK,Tapias V,D'Aiuto L,Chowdari KV,Francis L,Zhi Y,Ghosh BA,Surti U,Tischfield J,Sheldon M,Moore JC,Fish K,Nimgaonkar V

PA1-10005 was used in immunocytochemistry to discuss the consequences of copy number variation on chromosome 15q11.2

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: AC091465.1; DKFZp686I2148; MAP-2; map-2a; map2ab; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143

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Gene Aliases: G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4

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UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146

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Entrez Gene ID: (Human) 4133, (Mouse) 17756, (Rat) 25595

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Function(s)

dystroglycan binding structural molecule activity protein binding calmodulin binding microtubule binding cytoskeletal regulatory protein binding tubulin binding

Process(es)

microtubule bundle formation axonogenesis dendrite development cellular response to organic substance microtubule cytoskeleton organization central nervous system neuron development neuron projection development dendrite morphogenesis response to estradiol response to progesterone

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MAP2 Polyclonal Antibody

MAP2 Antibody (PA1-10005) in ICC/IF

Product Details

PA1-10005

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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