Invitrogen

MAP2 Polyclonal Antibody

MAP2 Antibody in Immunocytochemistry (ICC/IF)

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MAP2 Antibody (PA1-16751) in ICC/IF

Immunocytochemistry analysis of MAP2 in cortical neuron-glial cell culture from E20 rat. Samples were incubated in MAP2 polyclonal antibody (Product # PA1-16751) using a dilution of 1:10000. This antibody in red, and mouse mAb to MAP-tau dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. This antibody stains dendrites and perikarya of neurons, while Tau antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, wh... View More

MAP2 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-16751

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:20,000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

1:1,000-1:5,000

Immunocytochemistry (ICC/IF)

1:5,000-1:10,000

View 3 publications 3 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Chimpanzee, Human, Mouse, Non-human primate, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human construct of projection domain sequences, amino acds 377-1505

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

IgY fraction

Storage buffer

PBS

Contains

0.035% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2138189

Product Specific Information

In Western blot bands can be seen at approximately 280 kDa, representing MAP2a and MAP2b.

Bands may also be seen at approximately 70 kDa, representing MAP2c.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pervasive compartment-specific regulation of gene expression during homeostatic synaptic scaling.

EMBO reports

Colameo D,Rajman M,Soutschek M,Bicker S,von Ziegler L,Bohacek J,Winterer J,Germain PL,Dieterich C,Schratt G

PA1-16751 was used in Proximity Ligation Assay (PLA) and Immunohistochemistry to indicate that, during synaptic scaling, compartmentalized gene expression changes might co-exist with neuron-wide mechanisms to allow synaptic computation and homeostasis.

Tue Oct 05 00:00:00 EDT 2021

Pervasive compartment-specific regulation of gene expression during homeostatic synaptic scaling.

EMBO reports

Colameo D,Rajman M,Soutschek M,Bicker S,von Ziegler L,Bohacek J,Winterer J,Germain PL,Dieterich C,Schratt G

Tue Oct 05 00:00:00 EDT 2021

Capping Protein Regulator and Myosin 1 Linker 3 (CARMIL3) as a Molecular Signature of Ischemic Neurons in the DWI-T2 Mismatch Areas After Stroke.

Frontiers in molecular neuroscience

Yeh SJ,Hsu PH,Yeh TY,Yang WK,Chang KP,Chiang CS,Tang SC,Tsai LK,Jeng JS,Hsieh ST

PA1-16751 was used in Immunocytochemistry to explore (1) the protein expression profiles in the T2(+), mismatch, and contralateral areas, and (2) the protein with the highest expression in the T2(+) area in the brains of male Sprague-Dawley rats within 4.5 h after middle cerebral artery occlusion (MCAO).

Tue Jan 04 00:00:00 EST 2022

Capping Protein Regulator and Myosin 1 Linker 3 (CARMIL3) as a Molecular Signature of Ischemic Neurons in the DWI-T2 Mismatch Areas After Stroke.

Frontiers in molecular neuroscience

Yeh SJ,Hsu PH,Yeh TY,Yang WK,Chang KP,Chiang CS,Tang SC,Tsai LK,Jeng JS,Hsieh ST

Tue Jan 04 00:00:00 EST 2022

Structure-Activity Relationship of Neuroactive Steroids, Midazolam, and Perampanel Toward Mitigating Tetramine-Triggered Activity in Murine Hippocampal Neuronal Networks.

Toxicological sciences : an official journal of the Society of Toxicology

Antrobus S,Pressly B,Nik AM,Wulff H,Pessah IN

PA1-16751 was used in Immunocytochemistry to conclude that combinations of allopregnanolone and midazolam were significantly more effective at normalizing TETS-triggered SCO patterns, ESA patterns, and more potently enhanced GABA-activated Cl- current, than either drug alone.

Mon Apr 12 00:00:00 EDT 2021

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

PA1-16751 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

PA1-16751 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

PA1-16751 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

PA1-16751 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Human Cerebrospinal Fluid Monoclonal LGI1 Autoantibodies Increase Neuronal Excitability.

Annals of neurology

Kornau HC,Kreye J,Stumpf A,Fukata Y,Parthier D,Sammons RP,Imbrosci B,Kurpjuweit S,Kowski AB,Fukata M,Prüss H,Schmitz D

PA1-16751 was used in Immunohistochemistry to investigate the B-cell autoimmune response to leucine-rich glioma-inactivated 1 (LGI1) encephalitis.

Sun Mar 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: AC091465.1; DKFZp686I2148; MAP-2; map-2a; map2ab; Microtubule-associated protein 2; OTTHUMP00000163916; OTTHUMP00000208143

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Gene Aliases: G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4

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UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146

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Entrez Gene ID: (Human) 4133, (Mouse) 17756, (Rat) 25595

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Function(s)

dystroglycan binding structural molecule activity protein binding calmodulin binding microtubule binding cytoskeletal regulatory protein binding tubulin binding

Process(es)

microtubule bundle formation axonogenesis dendrite development cellular response to organic substance microtubule cytoskeleton organization central nervous system neuron development neuron projection development dendrite morphogenesis response to estradiol response to progesterone

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MAP2 Polyclonal Antibody

MAP2 Antibody (PA1-16751) in ICC/IF

Product Details

PA1-16751

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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