Invitrogen

MAP2 Monoclonal Antibody (AP20)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

9 References

View all (71) MAP2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MAP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

MAP2 Antibody (MA1-25044) in ICC/IF

Immunofluorescence analysis of Microtubule-associated protein 2 was performed using 70% confluent log phase SH-SY5Y cells differentiated to neurons (Retinoic acid, 10 uM for 5days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MAP2 Monoclonal Antibody (AP20) (Product # MA1-25044) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-... View More

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MAP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Increased expression of Microtubule-associated protein 2 was observed in SH-SY5Y differentiated to neurons (Retinoic acid, 10 uM for 5 days) compared to untreated SH-SY5Y using Anti-MAP2 Monoclonal Antibody (AP20) (Product # MA1-25044) in Western Blot. Also the same was used to observe higher expression of MAP2 in IMR-32 compared to BJ and BeWo. {RE}

Product Details

MA1-25044

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Amphibian, Avian, Bovine, Human, Mouse, Rat, Xenopus

Published species

Guinea pig, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AP20

Immunogen

Bovine protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.6 mg/mL

Storage buffer

Ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_794874

Product Specific Information

Recommended positive controls: rat cerebral cortex.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bulbar projecting subcortical GABAergic neurons send collateral branches extensively and selectively to primary olfactory cortical regions.

The Journal of comparative neurology

Hook C,Puche AC

MA1-25044 was used in Immunohistochemistry-immunofluorescence to reveal that bulbar projecting GABAergic neurons show remarkable specificity targeting other primary olfactory cortical regions exhibiting abundant collateral projections into the accessory olfactory bulb, AON, PFC, PC, and TT.

Wed Feb 01 00:00:00 EST 2023

Latent Tri-lineage Potential of Human Menstrual Blood-Derived Mesenchymal Stromal Cells Revealed by Specific In Vitro Culture Conditions.

Molecular neurobiology

Quintero-Espinosa D,Soto-Mercado V,Quintero-Quinchia C,Mendivil-Perez M,Velez-Pardo C,Jimenez-Del-Rio M

MA1-25044 was used in Western Blot to show that MenSCs are a reliable source to obtain functional neurogenic cells to further investigate the neurobiology of neurologic disorders.

Fri Oct 01 00:00:00 EDT 2021

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

MA1-25044 was used in Immunocytochemistry-immunoflourescence to develop a feasible, efficient, and reproducible method for the neural induction of mesenchymal stem cells isolated from Wharton's jelly (hWJ-MSCs).

Tue Mar 17 00:00:00 EDT 2020

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

Tue Mar 17 00:00:00 EDT 2020

Cholinergic-like neurons carrying PSEN1 E280A mutation from familial Alzheimer's disease reveal intraneuronal sAPPβ fragments accumulation, hyperphosphorylation of TAU, oxidative stress, apoptosis and Ca2+ dysregulation: Therapeutic implications.

PloS one

Soto-Mercado V,Mendivil-Perez M,Velez-Pardo C,Lopera F,Jimenez-Del-Rio M

MA1-25044 was used in Immunocytochemistry-immunoflourescence to demonstrate that FAD MSC-derived cholinergic neurons with the PSEN1 E280A mutation provide important clues for the identification of targetable pathological molecules.

Wed Sep 09 00:00:00 EDT 2020

PloS one

Soto-Mercado V,Mendivil-Perez M,Velez-Pardo C,Lopera F,Jimenez-Del-Rio M

Wed Sep 09 00:00:00 EDT 2020

Abnormal serine phosphorylation of insulin receptor substrate 1 is associated with tau pathology in Alzheimer's disease and tauopathies.

Acta neuropathologica

Yarchoan M,Toledo JB,Lee EB,Arvanitakis Z,Kazi H,Han LY,Louneva N,Lee VM,Kim SF,Trojanowski JQ,Arnold SE

MA1-25044 was used in immunohistochemistry - paraffin section to determine the expression of abnormal serine phosphorylation of insulin receptor substrate 1 in healthy and diseased human brains

Sat Nov 01 00:00:00 EDT 2014

ABCA1 influences neuroinflammation and neuronal death.

Neurobiology of disease

Karasinska JM,de Haan W,Franciosi S,Ruddle P,Fan J,Kruit JK,Stukas S,Lütjohann D,Gutmann DH,Wellington CL,Hayden MR

MA1-25044 was used in immunohistochemistry - paraffin section to elucidate the cell-type specific role of ABCA in neuroinflammation in vivo.

Sat Jun 01 00:00:00 EDT 2013

Transcriptional elongation factor elongin A regulates retinoic acid-induced gene expression during neuronal differentiation.

Cell reports

Yasukawa T,Bhatt S,Takeuchi T,Kawauchi J,Takahashi H,Tsutsui A,Muraoka T,Inoue M,Tsuda M,Kitajima S,Conaway RC,Conaway JW,Trainor PA,Aso T

MA1-25044 was used in immunocytochemistry to study retinoic acid-induced gene expression during neuronal differentiation and the role played by elongin A

Thu Nov 29 00:00:00 EST 2012

Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.

Developmental dynamics : an official publication of the American Association of Anatomists

Okae H,Iwakura Y

MA1-25044 was used in immunohistochemistry to study the effect of Gbeta1-deficiency on neural tube and neural progenitor cell proliferation

Thu Apr 01 00:00:00 EDT 2010

Immunolocalization of MAP-2 in routinely formalin-fixed, paraffin-embedded guinea pig brain sections using microwave irradiation: a comparison of different combinations of antibody clones and antigen retrieval buffer solutions.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

Kan RK,Pleva CM,Hamilton TA,Petrali JP

MA1-25044 was used in immunohistochemistry to study the efficacy of different microwave pre-treatments to restore MAP-2 immunoreactivity in fixed tissue preparations

Fri Apr 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: AC091465.1; DKFZp686I2148; MAP-2; map-2a; map2ab; microtubule associated protein 2C; Microtubule-associated protein 2; microtubule-associated protein-2; OTTHUMP00000163916; OTTHUMP00000208143

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Gene Aliases: a730034c02; G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4; XELAEV_18047318mg; xmap2

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UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146

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Entrez Gene ID: (Human) 4133, (Bovine) 281294, (Mouse) 17756, (Rat) 25595, (Xenopus) 379931

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Function(s)

dystroglycan binding structural molecule activity protein binding calmodulin binding microtubule binding cytoskeletal regulatory protein binding tubulin binding

Process(es)

microtubule bundle formation axonogenesis dendrite development cellular response to organic substance microtubule cytoskeleton organization central nervous system neuron development neuron projection development dendrite morphogenesis response to estradiol response to progesterone

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MAP2 Monoclonal Antibody (AP20)

MAP2 Antibody (MA1-25044) in ICC/IF

Product Details

MA1-25044

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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