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MAP4K2 Antibody (PA5-100733) in ICC/IF
Immunocytochemistry analysis of MAP4K2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MAP4K2 polyclonal antibody (Product # PA5-100733) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100733
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAP4K2(Accession Q12851), corresponding to amino acid residues E206-K256.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850236
Product Specific Information
Antibody detects endogenous levels of total MAP4K2.
Target Information
MAP4K2 (GCK) is a serine/ threonine protein kinase that activates the JNK pathway. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of the germinal center, where it may participate in B-cell differentiation. The kinase may also play a role in the regulation of vesicle targeting or fusion. The protein encoded by MAP4K2 is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphocyte serine/threonine protein kinase; B lymphocyte serine/threonine-protein kinase; GC kinase; Germinal center kinase; germinal centre kinase (GC kinase); MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; Mitogen-activated protein kinase kinase kinase kinase 2; Rab8 interacting protein; Rab8-interacting protein
Gene Aliases: BL44; GCK; MAP4K2; RAB8IP
UniProt ID: (Human) Q12851
Entrez Gene ID: (Human) 5871
Performance Guarantee
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