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MAP4K2 Antibody (PA5-109934) in WB
Western blot analysis of MAP4K2 in extracts of various cell lines. Samples were incubated with MAP4K2 Polyclonal antibody (Product # PA5-109934) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-109934
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 551-820 of human MAP4K2 (NP_004570.2).
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855345
Product Specific Information
Immunogen sequence: WAHDLPGLFE QRRLQQQVPL SIPTNRLTQR IIPRRFALST KIPDTKGCLQ CRVVRNPYTG ATFLLAALPT SLLLLQWYEP LQKFLLLKNF SSPLPSPAGM LEPLVLDGKE LPQVCVGAEG PEGPGCRVLF HVLPLEAGLT PDILIPPEGI PGSAQQVIQV DRDTILVSFE RCVRIVNMQG EPTATLAPEL TFDFPIETVV CLQDSVLAFW SHGMQGRSLD TNEVTQEITD ETRIFRVLGA HRDIILESIP TDNPEAHSNL YILTGHQSTY
Target Information
MAP4K2 (GCK) is a serine/ threonine protein kinase that activates the JNK pathway. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of the germinal center, where it may participate in B-cell differentiation. The kinase may also play a role in the regulation of vesicle targeting or fusion. The protein encoded by MAP4K2 is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphocyte serine/threonine protein kinase; B lymphocyte serine/threonine-protein kinase; GC kinase; GCK; Germinal center kinase; germinal centre kinase (GC kinase); MAPK/ERK kinase kinase kinase 2; MEK kinase kinase 2; MEKKK 2; mitogen activated protein kinase kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase kinase 2; RAB8 interacting protein; Rab8-interacting protein
Gene Aliases: AI385662; BL44; GCK; MAP4K2; RAB8IP
UniProt ID: (Human) Q12851, (Mouse) Q61161
Entrez Gene ID: (Human) 5871, (Rat) 293694, (Mouse) 26412
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
ATP binding
MAP kinase kinase kinase kinase activity
mitogen-activated protein kinase kinase kinase binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein phosphorylation
vesicle targeting
activation of JUN kinase activity
intracellular signal transduction
positive regulation of JNK cascade
MAPK cascade
activation of MAPKKK activity
immune system process
regulation of mitotic cell cycle
phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
innate immune response
immune response
JNK cascade
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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