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Invitrogen
MARCO Monoclonal Antibody (PLK-1), PE-Cyanine7, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
MARCO Antibody (25-5447-42) in Flow
Human monocyte derived macrophages were stimulated with either IFNg and LPS (left) or IL-10 (right) for 48 hours, and subsequently stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and MARCO Monoclonal Antibody, PE-Cyanine7. Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
25-5447-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
PLK-1
Immunogen
Human alveolar macrophages
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762452
Product Specific Information
Description: This PLK-1 monoclonal antibody recognizes human MARCO also known as SR-A6 or SCARA2.
Applications Reported: This PLK-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This PLK-1 antibody has been pre-diluted and tested by flow cytometric analysis of human monocyte-derived macrophages. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
MARCO, also known as SR-A6 or SCARA2, is a macrophage surface receptor and a member of the class A scavenger receptor family. As a part of the innate antimicrobial immune system, MARCO can bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. Due to its expression in tumor infiltrating macrophages, and its role in inhibiting immune responses within tumor environment, MARCO has been indicated as a potential target for cancer immunotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Macrophage receptor MARCO; Macrophage receptor with collagenous structure; Scavenger receptor class A member 2; scavenger receptor class A, member 2; SR-A6
Gene Aliases: MARCO; SCARA2
UniProt ID: (Human) Q9UEW3
Entrez Gene ID: (Human) 8685
Performance Guarantee
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