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MARK1 Antibody (PA5-29402) in WB
Western Blot using MARK1 Polyclonal Antibody (Product # PA5-29402). Sample (30 µg of whole cell lysate). Lane A: A431. 7.5% SDS PAGE. MARK1 Polyclonal Antibody (Product # PA5-29402) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-29402
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 265 of Human MARK1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546878
Product Specific Information
Recommended positive controls: A431, H1299, HeLa.
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (94%), Xenopus laevis (96%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MARK1 (MARK) is a serine/threonine kinase which is involved in cytoskeletal organization and biogenesis. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 is involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAP/microtubule affinity-regulating kinase 1; MARK1; Par-1c; PAR1 homolog c; Serine/threonine-protein kinase MARK1
Gene Aliases: KIAA1477; MARK; MARK1; Par-1c; Par1c
UniProt ID: (Human) Q9P0L2
Entrez Gene ID: (Human) 4139
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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