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FIGURE: 1 / 1
MAST2 Antibody (CF808830) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (RC209492, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2. Positive lysates LY402408 (100 µg) and LC402408 (20 µg) can be purchased separately from OriGene.
Product Details
CF808830
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2A3
Immunogen
Human recombinant protein fragment corresponding to amino acids 1482-1798 of human MAST2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Syntrophin is an adapter protein that functions to bind certain signaling molecules to the dystrophin-associated protein complex. This complex connects the extracellular matrix to the intracellular cytoskeleton for construction and maintenance of the postsynaptic structures in the neuromuscular junction and the central nervous system. Microtubule-associated serine/threonine-protein kinase 2 (MAST205) is a testis-specific, cytoplasmic protein that functions in a multi-protein complex in the maturation of spermatids. MAST205 is involved in linking the dystrophin/utrophin network with microtubule filaments via Syntrophin. By forming a complex with TRAF6, MAST205 regulates lipopolysaccharide-induced IL-12 synthesis in macrophages. This leads to the inhibition of TRAF6 NF-kappa-B activation. Two isoforms exist for MAST205 due to alternative splicing. Isoform 1 represents the full length protein, while isoform 2 lacks the residues 327-396 and 1091-1113. The N-terminus of MAST205 must be phosphorylated in order for ubiquitination to occur at the same site. This ubiquitination leads to the degradation of MAST205 via proteasome-mediated proteolysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mast2; microtubule associated testis specific serine/threonine protein kinase; Microtubule-associated serine/threonine-protein kinase 2
Gene Aliases: KIAA0807; MAST2; MAST205; MTSSK
UniProt ID: (Human) Q6P0Q8, (Mouse) Q60592
Entrez Gene ID: (Human) 23139, (Mouse) 17776, (Rat) 313819
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
microtubule binding
phosphatase binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
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