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Invitrogen
MAVS Monoclonal Antibody (ABM28H9), eFluor™ 450, eBioscience™
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
MAVS Antibody (48-9835-42) in Flow
HEL cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG2b kappa Isotype Control, eFluor 450 (Product # 48-4732-82) (blue histogram) or MAVS Monoclonal Antibody, eFluor 450 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 660 (Product # 65-0864-18).
Product Details
48-9835-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
ABM28H9
Immunogen
Recombinant fragment of human MAVS (aa 72-296)
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815186
Product Specific Information
Description: This ABM28H9 monoclonal antibody recognizes human MAVS, a mitochondrial membrane protein also known as VISA, CARDIF or IPS-1.
Applications Reported: This ABM18H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This ABM18H9 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of HEL cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. MAVS is a mitochondrial membrane protein that was identified as a critical component in the IFN beta signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappa-B. MAVS is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. MAVS also interacts with the IKK-alpha, IKK-beta and IKK-iota kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. Multiple isoforms of MAVS are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD adapter inducing interferon beta; CARD adaptor inducing IFN-beta; Cardif; IFN-B promoter stimulator 1; Interferon beta promoter stimulator protein 1; IPS-1; MAVS; Mitochondrial antiviral-signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adaptor; Virus-induced-signaling adapter; VISA
Gene Aliases: CARDIF; IPS-1; IPS1; KIAA1271; MAVS; VISA
UniProt ID: (Human) Q7Z434
Entrez Gene ID: (Human) 57506
positive regulation of protein phosphorylation
activation of innate immune response
positive regulation of defense response to virus by host
signal transduction
negative regulation of type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of protein import into nucleus, translocation
RIG-I signaling pathway
defense response to bacterium
positive regulation of transcription factor import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of viral genome replication
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
defense response to virus
positive regulation of type I interferon-mediated signaling pathway
cellular response to exogenous dsRNA
positive regulation of chemokine (C-C motif) ligand 5 production
positive regulation of IP-10 production
regulation of peroxisome organization
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