Invitrogen

MBL2 Monoclonal Antibody (3E7)

4 Published Figures

6 References

View all (19) MBL2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (4)

FIGURE: 1 / 4

MBL2 Antibody (MA1-40145) in Flow

Science advances 2023 - Fig. 2. MBL and hItln1 binding to microbial populations in human stool samples. ( A ) Schematic of flow cytometry-based assay to measure soluble lectin binding to stool microbiome. ( B ) Flow cytometry analysis of healthy human stool plotted as SYTO BC against lectin binding (anti-Strep DY649). Panels show no lectin control (column 1), and strep-MBL (top row), or strep-hItln1 (bottom row) binding in the pre... View More

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Product Details

MA1-40145

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:50

Flow Cytometry (Flow)

1:50

View 5 publications 5 publications

ELISA (ELISA)

1:50

Functional Assay (Functional)

1:50

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E7

Immunogen

Serum isolated human RaRF

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076469

Product Specific Information

MA1-40145 detects MBL from human samples.

The MA1-40145 immunogen is Mannose Binding Lectin (MBL).

MA1-40145 has been successfully used in flow cytometry, Western blot, ELISA, Functional Assays, and immunohistochemistry (frozen) applications.

Negative control: Nonimmune normal serum or TBS.

Endotoxin <24 EU/mg.

Target Information

MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lectin-Seq: A method to profile lectin-microbe interactions in native communities.

Science advances

McPherson RL,Isabella CR,Walker RL,Sergio D,Bae S,Gaca T,Raman S,Nguyen LTT,Wesener DA,Halim M,Wuo MG,Dugan A,Kerby R,Ghosh S,Rey FE,Dhennezel C,Pishchany G,Lensch V,Vlamakis H,Alm EJ,Xavier RJ,Kiessling LL

Published figure using MBL2 monoclonal antibody (Product # MA1-40145) in Flow Cytometry

Fri Jul 28 00:00:00 EDT 2023

The classical pathway triggers pathogenic complement activation in membranous nephropathy.

Nature communications

Seifert L,Zahner G,Meyer-Schwesinger C,Hickstein N,Dehde S,Wulf S,Köllner SMS,Lucas R,Kylies D,Froembling S,Zielinski S,Kretz O,Borodovsky A,Biniaminov S,Wang Y,Cheng H,Koch-Nolte F,Zipfel PF,Hopfer H,Puelles VG,Panzer U,Huber TB,Wiech T,Tomas NM

MA1-40145 was used in Proximity Ligation Assay (PLA) to show that, in MN, complement is primarily activated via the classical pathway and targeting complement components such as C3 may represent a promising therapeutic strategy.

Sat Jan 28 00:00:00 EST 2023

Rapid Transduction and Expansion of Transduced T Cells with Maintenance of Central Memory Populations.

Molecular therapy. Methods & clinical development

Pampusch MS,Haran KP,Hart GT,Rakasz EG,Rendahl AK,Berger EA,Connick E,Skinner PJ

Published figure using MBL2 monoclonal antibody (Product # MA1-40145) in Flow Cytometry

Fri Mar 13 00:00:00 EDT 2020

Simian Immunodeficiency Virus (SIV)-Specific Chimeric Antigen Receptor-T Cells Engineered to Target B Cell Follicles and Suppress SIV Replication.

Frontiers in immunology

Haran KP,Hajduczki A,Pampusch MS,Mwakalundwa G,Vargas-Inchaustegui DA,Rakasz EG,Connick E,Berger EA,Skinner PJ

MA1-40145 was used in Flow cytometry/Cell sorting to show that antiviral CAR/CXCR5-expressing T cells when infused into an SIV-infected animal or an HIV-infected individual, will home to B cell follicles suppress viral replication and lead to long-term durable remission of SIV and HIV.

Wed Apr 10 00:00:00 EDT 2019

Commercially available complement component-depleted sera are unexpectedly codepleted of ficolin-2.

Clinical and vaccine immunology : CVI

Brady AM,Geno KA,Dalecki AG,Cheng X,Nahm MH

Published figure using MBL2 monoclonal antibody (Product # MA1-40145) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Blood collection tubes influence serum ficolin-1 and ficolin-2 levels.

Clinical and vaccine immunology : CVI

Brady AM,Spencer BL,Falsey AR,Nahm MH

MA1-40145 was used in flow cytometry to study the effect of different blood collection tubes on the measured levels of serum ficolin-1 and -2

Wed Jan 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Collectin-1; Collectin1; mannan-binding lectin; Mannan-binding protein; Mannose-binding lectin; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); Mannose-binding protein C; MBP-C; MBP1; MBPC; MGC116832; MGC116833

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Gene Aliases: COLEC1; HSMBPC; MBL; MBL2; MBL2D; MBP; MBP-C; MBP1; MBPD

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UniProt ID: (Human) P11226

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Entrez Gene ID: (Human) 4153

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Function(s)

serine-type endopeptidase activity receptor binding calcium ion binding protein binding mannose binding calcium-dependent protein binding surfactant

Process(es)

complement activation, lectin pathway proteolysis acute-phase response complement activation complement activation, classical pathway response to oxidative stress opsonization defense response to bacterium negative regulation of growth of symbiont in host innate immune response negative regulation of viral process positive regulation of phagocytosis defense response to Gram-positive bacterium killing by host of symbiont cells

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MBL2 Monoclonal Antibody (3E7)

MBL2 Antibody (MA1-40145) in Flow

Product Details

MA1-40145

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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