Invitrogen

MBP Polyclonal Antibody

MBP Antibody in Immunocytochemistry (ICC/IF)

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MBP Antibody (PA1-10008) in ICC/IF

Immunofluorescent analysis of MBP cortical neuron-glial cell culture. The culture was prepared from an E20 rat and stained using an MBP polyclonal antibody (Product # PA1-10008) at a dilution of 1:2,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The antibody stains developing oligodendrocytes, cells, that form myelin around axons in the central nerve system.

MBP Antibody in Immunohistochemistry (IHC)

MBP Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

PA1-10008

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000

Immunohistochemistry (IHC)

1:20,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:2,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Mouse, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Full length purified myelin basic protein isolated from cow nerve

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077024

Product Specific Information

In Western blot using rat spinal cord whole tissue homogenates, this antibody detects all isotypes of MPB, resulting in bands at ~21.5kDa, 18.5kDa, 17kDa and 14kDa. |Stable at 4°C for at least one year.

Target Information

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Local Administration of Minocycline Improves Nerve Regeneration in Two Rat Nerve Injury Models.

International journal of molecular sciences

Guillemot-Legris O,Girmahun G,Shipley RJ,Phillips JB

PA1-10008 was used in Immunohistochemistry to test the effects of minocycline on SCL 4.1/F7 Schwann cells in vitro and on sciatic nerve explants.

Fri Jul 28 00:00:00 EDT 2023

Myelin insulation as a risk factor for axonal degeneration in autoimmune demyelinating disease.

Nature neuroscience

Schäffner E,Bosch-Queralt M,Edgar JM,Lehning M,Strauß J,Fleischer N,Kungl T,Wieghofer P,Berghoff SA,Reinert T,Krueger M,Morawski M,Möbius W,Barrantes-Freer A,Stieler J,Sun T,Saher G,Schwab MH,Wrede C,Frosch M,Prinz M,Reich DS,Flügel A,Stadelmann C,Fledrich R,Nave KA,Stassart RM

PA1-10008 was used in Immunohistochemistry to demonstrate that myelin ensheathment itself becomes detrimental for axonal survival and increases the risk of axons degenerating in an autoimmune environment.

Sat Jul 01 00:00:00 EDT 2023

Tunable Conductive Hydrogel Scaffolds for Neural Cell Differentiation.

Advanced healthcare materials

Tringides CM,Boulingre M,Khalil A,Lungjangwa T,Jaenisch R,Mooney DJ

PA1-10008 was used in Immunohistochemistry-immunofluorescence to show that as these materials have sufficient electrical properties to be used as electrodes in implantable arrays, they may allow for the creation of biohybrid neural interfaces and living electrodes.

Wed Mar 01 00:00:00 EST 2023

Enhanced liver X receptor signalling reduces brain injury and promotes tissue regeneration following experimental intracerebral haemorrhage: roles of microglia/macrophages.

Stroke and vascular neurology

Zhang R,Dong Y,Liu Y,Moezzi D,Ghorbani S,Mirzaei R,Lozinski BM,Dunn JF,Yong VW,Xue M

PA1-10008 was used in Immunohistochemistry-immunofluorescence to examine the benefits of enhanced Liver X receptor signalling in experimental intracerebral haemorrhage.

Fri Dec 29 00:00:00 EST 2023

Huntington disease oligodendrocyte maturation deficits revealed by single-nucleus RNAseq are rescued by thiamine-biotin supplementation.

Nature communications

Lim RG,Al-Dalahmah O,Wu J,Gold MP,Reidling JC,Tang G,Adam M,Dansu DK,Park HJ,Casaccia P,Miramontes R,Reyes-Ortiz AM,Lau A,Hickman RA,Khan F,Paryani F,Tang A,Ofori K,Miyoshi E,Michael N,McClure N,Flowers XE,Vonsattel JP,Davidson S,Menon V,Swarup V,Fraenkel E,Goldman JE,Thompson LM

PA1-10008 was used in Immunohistochemistry to identify cell type-specific and -agnostic signatures suggesting oligodendrocytes (OLs) and oligodendrocyte precursors (OPCs) are arrested in intermediate maturation states.

Wed Dec 21 00:00:00 EST 2022

Whole-Brain Mapping of the Expression Pattern of T1R2, a Subunit Specific to the Sweet Taste Receptor.

Frontiers in neuroanatomy

Jang JH,Kim HK,Seo DW,Ki SY,Park S,Choi SH,Kim DH,Moon SJ,Jeong YT

PA1-10008 was used in Immunohistochemistry-immunofluorescence to indicate that sweet taste receptors have unidentified functions in the brain and suggest that they may be a novel therapeutic target in the central nervous system.

Wed Nov 17 00:00:00 EST 2021

Whole-Brain Mapping of the Expression Pattern of T1R2, a Subunit Specific to the Sweet Taste Receptor.

Frontiers in neuroanatomy

Jang JH,Kim HK,Seo DW,Ki SY,Park S,Choi SH,Kim DH,Moon SJ,Jeong YT

Wed Nov 17 00:00:00 EST 2021

Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons.

Acta neuropathologica communications

Potratz M,Zaeck LM,Weigel C,Klein A,Freuling CM,Müller T,Finke S

Published figure using MBP polyclonal antibody (Product # PA1-10008) in Immunohistochemistry

Mon Nov 23 00:00:00 EST 2020

Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons.

Acta neuropathologica communications

Potratz M,Zaeck LM,Weigel C,Klein A,Freuling CM,Müller T,Finke S

Published figure using MBP polyclonal antibody (Product # PA1-10008) in Immunohistochemistry

Mon Nov 23 00:00:00 EST 2020

Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons.

Acta neuropathologica communications

Potratz M,Zaeck LM,Weigel C,Klein A,Freuling CM,Müller T,Finke S

Published figure using MBP polyclonal antibody (Product # PA1-10008) in Immunohistochemistry

Mon Nov 23 00:00:00 EST 2020

Regulation of later neurogenic stages of adult-derived neural stem/progenitor cells by L-type Ca2+ channels.

Development, growth & differentiation

Teh DB,Ishizuka T,Yawo H

PA1-10008 was used in immunocytochemistry to determine which stage of neurogenesis is under the regulation of L-type Ca2+ channels

Wed Oct 01 00:00:00 EDT 2014

Optogenetic patterning of whisker-barrel cortical system in transgenic rat expressing channelrhodopsin-2.

PloS one

Honjoh T,Ji ZG,Yokoyama Y,Sumiyoshi A,Shibuya Y,Matsuzaka Y,Kawashima R,Mushiake H,Ishizuka T,Yawo H

PA1-10008 was used in immunohistochemistry to develop a system that enables the precise spatiotemporal stimulation of the whisker-barrel cortical system using rats transgenically expressing channelrhodopsin-2

Mon Feb 02 00:00:00 EST 2015

Light-evoked somatosensory perception of transgenic rats that express channelrhodopsin-2 in dorsal root ganglion cells.

PloS one

Ji ZG,Ito S,Honjoh T,Ohta H,Ishizuka T,Fukazawa Y,Yawo H

PA1-10008 was used in immunohistochemistry to study light-evoked somatosensory perception in transgenic rats expressing channelrhodopsin-2

Thu Aug 02 00:00:00 EDT 2012

Light-evoked somatosensory perception of transgenic rats that express channelrhodopsin-2 in dorsal root ganglion cells.

PloS one

Ji ZG,Ito S,Honjoh T,Ohta H,Ishizuka T,Fukazawa Y,Yawo H

PA1-10008 was used in immunohistochemistry to study light-evoked somatosensory perception in transgenic rats expressing channelrhodopsin-2

Thu Aug 02 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 20 kDa microtubule-stabilizing protein; golli mbp; Golli-Mbp; myelin basic protein; Golli-Mbp; myelin basic protein; myelin basic protein S; MBP; MBP S; MGC99675; microtubule-stabilizing protein; Myelin A1 protein; Myelin basic protein; myelin basic protein Golli-mbp; myelin basic protein S; myelin deficient; Myelin membrane encephalitogenic protein; shiverer

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Gene Aliases: C76307; golli-mbp; Hmbpr; MBP; Mbps; mld; R75289; Shi

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UniProt ID: (Human) P02686, (Rat) P02688, (Mouse) P04370

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Entrez Gene ID: (Human) 4155, (Pig) 414286, (Bovine) 618684, (Rat) 24547, (Mouse) 17196

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Function(s)

protease binding protein binding structural constituent of myelin sheath calmodulin binding phospholipid binding myelin protein structural protein

Process(es)

immune response chemical synaptic transmission central nervous system development aging axon ensheathment response to toxic substance substantia nigra development response to progesterone response to tumor necrosis factor myelination response to mercury ion negative regulation of axonogenesis membrane organization response to fatty acid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MBP Polyclonal Antibody

MBP Antibody (PA1-10008) in ICC/IF

Product Details

PA1-10008

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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