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MBP Antibody (PA1-18324) in WB
Fluids and barriers of the CNS 2013 -
Figure 3 Western blot analyses for myelin basic protein (MBP). a . Western blots for MBP . Ten-lane precast gels were used. Each lane had an individual 5 mul sample of CSF from the categories of patients shown. After protein separation using SDS PAGE they were blotted onto a PVDF membrane for probing with antibodies. ECL detection was used to visualise the labelled bands and for semi-quantitative analysis u... View More
Product Details
PA1-18324
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Guinea pig,
Human,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues Y(68) G S L P Q K S Q R S Q D E N P V V(86) of guinea pig MBP, conjugated to KLH.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1077023
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Target Information
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: golli mbp; Golli-Mbp; myelin basic protein; Golli-Mbp; myelin basic protein; myelin basic protein S; MBP; MBP S; MGC99675; Myelin A1 protein; Myelin basic protein; myelin basic protein Golli-mbp; myelin basic protein S; Myelin membrane encephalitogenic protein
Gene Aliases: MBP; Mbps
UniProt ID: (Human) P02686, (Rat) P02688
Entrez Gene ID: (Human) 4155, (Guinea pig) 100731253, (Rat) 24547
immune response
chemical synaptic transmission
central nervous system development
aging
axon ensheathment
response to toxic substance
substantia nigra development
response to progesterone
response to tumor necrosis factor
myelination
response to mercury ion
negative regulation of axonogenesis
membrane organization
response to fatty acid
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