Invitrogen

CD45 Monoclonal Antibody (30-F11), Pacific Blue™

5 Published Figures

19 References

View all (407) CD45 antibodies

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

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CD45 Antibody (MCD4528) in Flow

Balb/c mouse spleen cells were stained with Pacific Blue™-conjugated anti-mouse CD45 (Product # MCD4528). The negative profile represents unstained cells.

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Product Details

MCD4528

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

30-F11

Immunogen

The CD45 antigen

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373710

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

USP10 is a potential mediator for vagus nerve stimulation to alleviate neuroinflammation in ischaemic stroke by inhibiting NF-κB signalling pathway.

Frontiers in immunology

Xie C,Gao X,Liu G,Tang H,Li C

MCD4528 was used in Flow cytometry/Cell sorting to investigate whether USP10 plays a key role in the protective effect of VNS against ischemic stroke and explore its mechanism.

Thu May 18 00:00:00 EDT 2023

Adipocyte-Secreted IL-6 Sensitizes Macrophages to IL-4 Signaling.

Diabetes

Luan D,Dadpey B,Zaid J,Bridge-Comer PE,DeLuca JH,Xia W,Castle J,Reilly SM

MCD4528 was used in Flow Cytometry to investigate catabolic stimulation of adipocyte IL-6 secretion and its impact on adipose tissue immune cells.

Wed Mar 01 00:00:00 EST 2023

Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy.

Frontiers in cell and developmental biology

Parker E,Khayrullin A,Kent A,Mendhe B,Youssef El Baradie KB,Yu K,Pihkala J,Liu Y,McGee-Lawrence M,Johnson M,Chen J,Hamrick M

MCD4528 was used in Flow Cytometry to investigate the role of FAPs in disuse atrophy in vivo utilizing a 2-week single hindlimb immobilization model.

Fri Jan 21 00:00:00 EST 2022

IL-1α Mediates Innate and Acquired Resistance to Immunotherapy in Melanoma.

Journal of immunology (Baltimore, Md. : 1950)

Singh S,Xiao Z,Bavisi K,Roszik J,Melendez BD,Wang Z,Cantwell MJ,Davis RE,Lizee G,Hwu P,Neelapu SS,Overwijk WW,Singh M

MCD4528 was used in Flow Cytometry to suggest that blocking the IL-1α signaling pathway may increase the efficacy of immunotherapies against melanoma.

Thu Apr 15 00:00:00 EDT 2021

Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model.

International journal of molecular sciences

Hong M,Han IH,Choi I,Cha N,Kim W,Kim SK,Bae H

MCD4528 was used in Flow cytometry/Cell sorting to investigate whether the oral administration of M.C could decrease cisplatin-induced sarcopenia by modulating M2 macrophage polarization in mice.

Sat Mar 20 00:00:00 EDT 2021

Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model.

International journal of molecular sciences

Hong M,Han IH,Choi I,Cha N,Kim W,Kim SK,Bae H

MCD4528 was used in Flow cytometry/Cell sorting to investigate whether the oral administration of M.C could decrease cisplatin-induced sarcopenia by modulating M2 macrophage polarization in mice.

Sat Mar 20 00:00:00 EDT 2021

Tendon and motor phenotypes in the mouse model of recessive osteogenesis imperfecta.

eLife

Grol MW,Haelterman NA,Lim J,Munivez EM,Archer M,Hudson DM,Tufa SF,Keene DR,Lei K,Park D,Kuzawa CD,Ambrose CG,Eyre DR,Lee BH

MCD4528 was used in Flow Cytometry to show that mutant Achilles and patellar tendons were thinner and weaker with increased collagen cross-links and reduced collagen fibril size at 1- and 4-months compared to wildtype.

Wed May 26 00:00:00 EDT 2021

Tendon and motor phenotypes in the mouse model of recessive osteogenesis imperfecta.

eLife

Grol MW,Haelterman NA,Lim J,Munivez EM,Archer M,Hudson DM,Tufa SF,Keene DR,Lei K,Park D,Kuzawa CD,Ambrose CG,Eyre DR,Lee BH

Wed May 26 00:00:00 EDT 2021

Analyses of the pericyte transcriptome in ischemic skeletal muscles.

Stem cell research & therapy

Teng YC,Porfírio-Sousa AL,Ribeiro GM,Arend MC,da Silva Meirelles L,Chen ES,Rosa DS,Han SW

MCD4528 was used in Flow cytometry/Cell sorting to provide a database that will improve our understanding of skeletal muscle pericyte biology under ischemic conditions, which may be useful for the development of novel pericyte-based cell and gene therapies.

Tue Mar 16 00:00:00 EDT 2021

Lymph Node Stromal Cells Generate Antigen-Specific Regulatory T Cells and Control Autoreactive T and B Cell Responses.

Cell reports

Nadafi R,Gago de Graça C,Keuning ED,Koning JJ,de Kivit S,Konijn T,Henri S,Borst J,Reijmers RM,van Baarsen LGM,Mebius RE

MCD4528 was used in Flow Cytometry to uncover the ability of LNSCs to suppress the early activation of autoreactive immune cells and maintain peripheral tolerance.

Tue Mar 24 00:00:00 EDT 2020

A Subset of Skin Macrophages Contributes to the Surveillance and Regeneration of Local Nerves.

Immunity

Kolter J,Feuerstein R,Zeis P,Hagemeyer N,Paterson N,d'Errico P,Baasch S,Amann L,Masuda T,Lösslein A,Gharun K,Meyer-Luehmann M,Waskow C,Franzke CW,Grün D,Lämmermann T,Prinz M,Henneke P

MCD4528 was used in Flow cytometry/Cell sorting to delineate the postnatal development of dermal macrophages and their differentiation into subsets by adapting single-cell transcriptomics, fate mapping, and imaging.

Tue Jun 18 00:00:00 EDT 2019

Circadian variation in pulmonary inflammatory responses is independent of rhythmic glucocorticoid signaling in airway epithelial cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ince LM,Zhang Z,Beesley S,Vonslow RM,Saer BR,Matthews LC,Begley N,Gibbs JE,Ray DW,Loudon ASI

MCD4528 was used in Flow cytometry/Cell sorting to study the persistence of clock control of pulmonary neutrophilia without the requirement for rhythmic glucocorticoid availability.

Tue Jan 01 00:00:00 EST 2019

Genome-wide effect of pulmonary airway epithelial cell-specific Bmal1 deletion.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang Z,Hunter L,Wu G,Maidstone R,Mizoro Y,Vonslow R,Fife M,Hopwood T,Begley N,Saer B,Wang P,Cunningham P,Baxter M,Durrington H,Blaikley JF,Hussell T,Rattray M,Hogenesch JB,Gibbs J,Ray DW,Loudon ASI

MCD4528 was used in Flow Cytometry to find that metabolic cues dictate phasing of the pulmonary clock and circadian responses to immunologic challenges.

Wed May 01 00:00:00 EDT 2019

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS genetics

Madaro L,Torcinaro A,De Bardi M,Contino FF,Pelizzola M,Diaferia GR,Imeneo G,Bouchè M,Puri PL,De Santa F

Published figure using CD45 monoclonal antibody (Product # MCD4528) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Myeloid Cell-Derived HB-EGF Drives Tissue Recovery After Pancreatitis.

Cellular and molecular gastroenterology and hepatology

Wen HJ,Gao S,Wang Y,Ray M,Magnuson MA,Wright CVE,Di Magliano MP,Frankel TL,Crawford HC

MCD4528 was used in Flow cytometry/Cell sorting to demonstrate pancreas healing is supported by myeloid cell HB-EGF-induced epithelial cell proliferation and EGFR-dependent DNA repair.

Thu Jul 02 00:00:00 EDT 2020

PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration.

International journal of molecular sciences

Dammone G,Karaz S,Lukjanenko L,Winkler C,Sizzano F,Jacot G,Migliavacca E,Palini A,Desvergne B,Gilardi F,Feige JN

MCD4528 was used in Flow cytometry/Cell sorting to study muscle regeneration and tissue-resident muscle stem cell function in whole body Pparg null mice generated by epiblast-specific Cre/lox deletion (PpargΔ/Δ).

Fri Jul 13 00:00:00 EDT 2018

Platelet-activating factor (PAF) receptor as a promising target for cancer cell repopulation after radiotherapy.

Oncogenesis

da Silva IA,Chammas R,Lepique AP,Jancar S

MCD4528 was used in Flow cytometry/Cell sorting to propose that the PAF receptor (KBM) represents a possible target for improving the efficacy of radiotherapy through inhibition of tumor repopulation.

Mon Jan 30 00:00:00 EST 2017

Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice.

Nature medicine

Lukjanenko L,Jung MJ,Hegde N,Perruisseau-Carrier C,Migliavacca E,Rozo M,Karaz S,Jacot G,Schmidt M,Li L,Metairon S,Raymond F,Lee U,Sizzano F,Wilson DH,Dumont NA,Palini A,Fässler R,Steiner P,Descombes P,Rudnicki MA,Fan CM,von Maltzahn J,Feige JN,Bentzinger CF

MCD4528 was used in flow cytometry to show that the loss of stem cell adhesion to fibronectin is a mechanism of aging

Mon Aug 01 00:00:00 EDT 2016

Interleukin-10 From Marginal Zone Precursor B-Cell Subset Is Required for Costimulatory Blockade-Induced Transplantation Tolerance.

Transplantation

Lal G,Nakayama Y,Sethi A,Singh AK,Burrell BE,Kulkarni N,Brinkman CC,Iwami D,Zhang T,Bromberg JS

MCD4528 was used in flow cytometry to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance

Tue Sep 01 00:00:00 EDT 2015

Epithelial nuclear factor-κB signaling promotes lung carcinogenesis via recruitment of regulatory T lymphocytes.

Oncogene

Zaynagetdinov R,Stathopoulos GT,Sherrill TP,Cheng DS,McLoed AG,Ausborn JA,Polosukhin VV,Connelly L,Zhou W,Fingleton B,Peebles RS,Prince LS,Yull FE,Blackwell TS

MCD4528 was used in Flow cytometry/Cell sorting to study whether nuclear factor kP provides an interface between persistent lung inflammation and carcinogenesis.

Thu Jun 28 00:00:00 EDT 2012

Single cell analysis of complex thymus stromal cell populations: rapid thymic epithelia preparation characterizes radiation injury.

Clinical and translational science

Williams KM,Mella H,Lucas PJ,Williams JA,Telford W,Gress RE

MCD4528 was used in flow cytometry and immunocytochemistry to develop a new method to isolate thymic epithelial cells and study them after cytotoxic injury

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (30-F11), Pacific Blue™

CD45 Antibody (MCD4528) in Flow

Product Details

MCD4528

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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