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Product Details
66026-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1E3C2
Immunogen
MCL1 Fusion Protein Ag10609 (1-327 aa encoded by BC107735)
Conjugate
Form
Liquid
Concentration
1.21 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MFGLKRNAV IGLNLYCGGA GLGAGSGGAT RPGGRLLATE KEASARREIG GGEAGAVIGG SAGASPPSTL TPDSRRVARP PPIGAEVPDV TATPARLLFF APTRRAAPLE EMEAPAADAI MSPEEELDGY EPEPLGKRPA VLPLLELVGE SGNNTSTDGS LPSTPPPAEE EEDELYRQSL EIISRYLREQ ATGAKDTKPM GRSGATSRKA LETLRRVGDG VQRNHETAFQ GMLRKLDIKN EDDVKSLSRV MIHVFSDGVT NWGRIVTLIS FGAFVAKHLK TINQESCIEP LAESITDVLV RTKRDWLVKQ RGWDGFVEFF HVEDLEGG (1-327 aa encoded by BC107735)
Target Information
MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; Bcl2-L-3; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL-1S; mcl1/EAT; MGC104264; MGC1839; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)
Gene Aliases: bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
UniProt ID: (Human) D3DV04
Entrez Gene ID: (Human) 4170
cell fate determination
multicellular organism development
intrinsic apoptotic signaling pathway in response to DNA damage
apoptotic mitochondrial changes
cellular homeostasis
response to cytokine
negative regulation of apoptotic process
protein transmembrane transport
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
negative regulation of anoikis
regulation of response to DNA damage stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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