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MCP-1 Monoclonal Antibody, PeproTech®

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MCP-1 Antibody (500-M71-500UG) in WB

Western Blot: To detect Human MCP-1 (CCL2) by Western Blot analysis MCP-1 Monoclonal Antibody (Product # 500-M71-500UG) can be used at a concentration of 0.20-0.40 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-1 (CCL2) is 1.0-2.0 ng/lane under reducing conditions and 0.25-0.50 ng/lane under non-reducing conditions.

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Product Details

500-M71-500UG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.20-0.40 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

1.0-2.0 µg/mL

View 9 publications 9 publications

Neutralization (Neu)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human, Mouse

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MCP-1 (CCL2)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3074168

Product Specific Information

AA Sequence of recombinant protein: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified from cell culture supernatant by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 1.0-2.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 (CCL2) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-1 (CCL2) (500-P34BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.

Western Blot: To detect Human MCP-1 (CCL2) by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-1 (CCL2) is 1.0-2.0 ng/lane under reducing conditions and 0.25-0.50 ng/lane under non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Human Breast Cancer Are Mediated by PD-L1-S283 and Chemokine Axes.

Cancers

Erlichman N,Baram T,Meshel T,Morein D,Da'adoosh B,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to investigate the role of WT-PD-L1 in triple-negative breast cancer.

Fri Feb 18 00:00:00 EST 2022

Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Rubinstein-Achiasaf L,Morein D,Wiemann S,Körner C,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to study the impact of tumor-stroma-inflammation networks on pro-metastatic phenotypes in triple-negative breast cancer (TNBC) to identify a novel tumor-stroma-inflammation networks that may promote TNBC aggressiveness by increasing the pro-malignancy potential of the TME and of the tumor cells themselves, and reveal key roles for CXCL8 in mediating these metastasis-promoting activities.

Mon Aug 10 00:00:00 EDT 2020

Co-Inflammatory Roles of TGFβ1 in the Presence of TNFα Drive a Pro-inflammatory Fate in Mesenchymal Stem Cells.

Frontiers in immunology

Lerrer S,Liubomirski Y,Bott A,Abnaof K,Oren N,Yousaf A,Körner C,Meshel T,Wiemann S,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to demonstrate a TNFα + TGFβ1-driven pro-inflammatory fate in MSCs, identify specific molecular mechanisms involved, and propose that TNFα + TGFβ1-stimulated MSCs influence the tumor niche.

Wed Nov 20 00:00:00 EST 2019

M-sec regulates polarized secretion of inflammatory endothelial chemokines and facilitates CCL2-mediated lymphocyte transendothelial migration.

Journal of leukocyte biology

Barzilai S,Blecher-Gonen R,Barnett-Itzhaki Z,Zauberman A,Lebel-Haziv Y,Amit I,Alon R

500-M71 was used in Enzyme-linked immunosorbent assay to the first evidence of a novel inflammation-inducible machinery that regulates polarized secretion of endothelial CCL2 and other inflammatory chemokines and cytokines into basolateral endothelial compartments and facilitates the ability of endothelial CCL2 to promote T cell transendothelial migration.

Wed Jun 01 00:00:00 EDT 2016

Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-α and the NF-κB pathway.

Stem cell research & therapy

Katanov C,Lerrer S,Liubomirski Y,Leider-Trejo L,Meshel T,Bar J,Feniger-Barish R,Kamer I,Soria-Artzi G,Kahani H,Banerjee D,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to study the impact of tumour necrosis factor α and interleukin 1β on the inflammatory phenotype of cancer-associated fibroblasts and mesenchymal stem cells.

Fri May 01 00:00:00 EDT 2015

Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs.

Neoplasia (New York, N.Y.)

Lebel-Haziv Y,Meshel T,Soria G,Yeheskel A,Mamon E,Ben-Baruch A

500-M71 was used in Immunocytochemistry-immunoflourescence to propose that targeting these chemokine regions may lead to reduced secretion of CCL2 by breast cancer cells (and potentially also by other malignant cells).

Mon Sep 01 00:00:00 EDT 2014

The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells.

BMC cancer

Leibovich-Rivkin T,Liubomirski Y,Meshel T,Abashidze A,Brisker D,Solomon H,Rotter V,Weil M,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to determine the relative contribution of two processes to breast cancer progression: (1) Intrinsic events, such as activation of the Ras pathway and down-regulation of p53; (2) The inflammatory cytokines TNFα and IL-1β, shown in our published studies to be highly expressed in tumors of >80% of breast cancer patients with recurrent disease.

Thu Mar 06 00:00:00 EST 2014

Progression of luminal breast tumors is promoted by ménage à trois between the inflammatory cytokine TNFα and the hormonal and growth-supporting arms of the tumor microenvironment.

Mediators of inflammation

Weitzenfeld P,Meron N,Leibovich-Rivkin T,Meshel T,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to indicate that TNF α can turn into a strong prometastatic factor, suggesting a paradigm shift in which clinically approved inhibitors of TNFα would be applied in breast cancer therapy.

Thu Jun 05 00:00:00 EDT 2014

Inflammatory factors of the tumor microenvironment induce plasticity in nontransformed breast epithelial cells: EMT, invasion, and collapse of normally organized breast textures.

Neoplasia (New York, N.Y.)

Leibovich-Rivkin T,Liubomirski Y,Bernstein B,Meshel T,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to reveal novel pathways through which the inflammatory microenvironment may contribute to relapsed disease in breast cancer.

Sun Dec 01 00:00:00 EST 2013

Tumor-promoting circuits that regulate a cancer-related chemokine cluster: dominance of inflammatory mediators over oncogenic alterations.

Cancers

Leibovich-Rivkin T,Buganim Y,Solomon H,Meshel T,Rotter V,Ben-Baruch A

500-M71 was used in Enzyme-linked immunosorbent assay to use an unbiased model system to propose that inflammatory mediators of the tumor milieu have dominating roles over oncogenic modifications in dictating the expression of a pro-malignancy chemokine readout.

Fri Jan 20 00:00:00 EST 2012

Endogenous CCL2 (monocyte chemotactic protein-1) modulates human immunodeficiency virus type-1 replication and affects cytoskeleton organization in human monocyte-derived macrophages.

Blood

Fantuzzi L,Spadaro F,Vallanti G,Canini I,Ramoni C,Vicenzi E,Belardelli F,Poli G,Gessani S

Wed Oct 01 00:00:00 EDT 2003

Identification of human complement factor H as a chemotactic protein for monocytes.

The Biochemical journal

Nabil K,Rihn B,Jaurand MC,Vignaud JM,Ripoche J,Martinet Y,Martinet N

500-M71 was used in neutralization assay.

Mon Sep 01 00:00:00 EDT 1997

Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells.

Kidney international

Kakizaki Y,Waga S,Sugimoto K,Tanaka H,Nukii K,Takeya M,Yoshimura T,Yokoyama M

Fri Dec 01 00:00:00 EST 1995

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2

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Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF

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UniProt ID: (Human) P13500

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Entrez Gene ID: (Human) 6347

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Function(s)

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule

Process(es)

Performance Guarantee

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MCP-1 Monoclonal Antibody, PeproTech®

MCP-1 Antibody (500-M71-500UG) in WB

Product Details

500-M71-500UG

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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