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MCP-1 Antibody (500-P113-1MG) in WB
Western Blot: To detect Murine JE/MCP-1 (CCL2) by Western Blot analysis MCP-1 Polyclonal Antibody (Product # 500-P113-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine JE/MCP-1 (CCL2) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P113-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine JE/MCP-1 (CCL2)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929270
Product Specific Information
AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine JE/MCP-1 (CCL2). Anti-Murine JE/MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Murine JE/MCP-1 (CCL2) matrix.
Sandwich ELISA: To detect Murine JE/MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine JE/MCP-1 (CCL2) (500-P113Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine JE/MCP-1(CCL2).
Western Blot: To detect Murine JE/MCP-1 (CCL2) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine JE/MCP-1 (CCL2) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 2; H-MCP-1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; Small-inducible cytokine A2
Gene Aliases: AI323594; Ccl2; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF
UniProt ID: (Mouse) P10148
Entrez Gene ID: (Mouse) 20296
angiogenesis
positive regulation of leukocyte mediated cytotoxicity
positive regulation of endothelial cell proliferation
leukocyte migration involved in inflammatory response
positive regulation of leukocyte migration
positive regulation of cellular extravasation
cellular calcium ion homeostasis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
transforming growth factor beta receptor signaling pathway
glial cell migration
regulation of cell shape
response to heat
response to wounding
regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
negative regulation of angiogenesis
cytokine-mediated signaling pathway
positive regulation of cell-cell adhesion
neutrophil chemotaxis
positive regulation of tumor necrosis factor production
positive regulation of collagen biosynthetic process
helper T cell extravasation
monocyte homeostasis
positive regulation of cell adhesion
vascular endothelial growth factor receptor signaling pathway
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of synaptic transmission
positive regulation of T cell activation
positive regulation of nitric-oxide synthase biosynthetic process
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of monocyte chemotaxis
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of protein targeting to membrane
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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