Invitrogen

MCP-1 Polyclonal Antibody, Biotin, PeproTech®

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View all (67) CCL2 (MCP-1) antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

500-P113BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine JE/MCP-1 (CCL2)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929273

Product Specific Information

AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine JE/MCP-1 (CCL2). Anti-Murine JE/MCP-1 (CCL2)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Murine JE/MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine JE/MCP-1 (CCL2) (500-P113) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine JE/MCP-1 (CCL2).

Western Blot: To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mJE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P113BT-1MG will be provided as 2 x 500 µg

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 2; H-MCP-1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; Small-inducible cytokine A2

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Gene Aliases: AI323594; Ccl2; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF

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UniProt ID: (Mouse) P10148

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Entrez Gene ID: (Mouse) 20296

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Function(s)
G-protein coupled receptor binding cytokine activity protein binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule
Process(es)
angiogenesis positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response positive regulation of leukocyte migration positive regulation of cellular extravasation cellular calcium ion homeostasis chemotaxis inflammatory response immune response cytoskeleton organization transforming growth factor beta receptor signaling pathway glial cell migration regulation of cell shape response to heat response to wounding regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis negative regulation of angiogenesis cytokine-mediated signaling pathway positive regulation of cell-cell adhesion neutrophil chemotaxis positive regulation of tumor necrosis factor production positive regulation of collagen biosynthetic process helper T cell extravasation monocyte homeostasis positive regulation of cell adhesion vascular endothelial growth factor receptor signaling pathway eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of synaptic transmission positive regulation of T cell activation positive regulation of nitric-oxide synthase biosynthetic process chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of protein targeting to membrane positive regulation of apoptotic cell clearance
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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