Invitrogen

MCP-1 Polyclonal Antibody, Biotin, PeproTech®

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View all (67) CCL2 (MCP-1) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P76BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
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Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Rat MCP-1 (CCL2)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930753

Product Specific Information

AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MCP-1 (CCL2). Anti-Rat MCP-1 (CCL2)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat MCP-1(MCAF) (500-P76) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MCP-1(MCAF).

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P76BT-1MG will be provided as 2 x 500 µg

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 2; H-MCP-1; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; RP23-350G1.3; small inducible cytokine A2; small inducible gene JE; Small-inducible cytokine A2

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Gene Aliases: Ccl2; Je; MCP-1; Mcp1; Scya2; Sigje

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UniProt ID: (Rat) P14844

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Entrez Gene ID: (Rat) 24770

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Function(s)
G-protein coupled receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule
Process(es)
MAPK cascade response to hypoxia positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response monocyte chemotaxis positive regulation of leukocyte migration positive regulation of cellular extravasation cellular calcium ion homeostasis inflammatory response cytoskeleton organization transforming growth factor beta receptor signaling pathway aging glial cell migration regulation of cell shape response to heat response to wounding response to mechanical stimulus response to bacterium response to gamma radiation regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis response to activity negative regulation of angiogenesis cytokine-mediated signaling pathway positive regulation of cell-cell adhesion neutrophil chemotaxis organ regeneration lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to progesterone positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of collagen biosynthetic process response to vitamin B3 negative regulation of glial cell apoptotic process helper T cell extravasation cellular response to drug cellular response to macrophage colony-stimulating factor stimulus cellular response to platelet-derived growth factor stimulus chemokinesis response to drug response to amino acid protein kinase B signaling negative regulation of neuron apoptotic process astrocyte cell migration cellular response to fibroblast growth factor stimulus response to ethanol positive regulation of cell adhesion response to antibiotic vascular endothelial growth factor receptor signaling pathway macrophage chemotaxis lymphocyte chemotaxis positive regulation of synaptic transmission positive regulation of T cell activation response to glucocorticoid positive regulation of nitric-oxide synthase biosynthetic process maternal process involved in female pregnancy maternal process involved in parturition chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to ATP cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to lipoprotein particle stimulus cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of calcium ion import positive regulation of protein targeting to membrane positive regulation of apoptotic cell clearance negative regulation of natural killer cell chemotaxis
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