Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
MCP-1 Antibody (500-P76BT-25UG) in WB
Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis MCP-1 Polyclonal Antibody, Biotin (Product # 500-P76BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P76BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat MCP-1 (CCL2)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930753
Product Specific Information
AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MCP-1 (CCL2). Anti-Rat MCP-1 (CCL2)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat MCP-1(MCAF) (500-P76) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MCP-1(MCAF).
Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P76BT-1MG will be provided as 2 x 500 µg
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 2; H-MCP-1; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; RP23-350G1.3; small inducible cytokine A2; small inducible gene JE; Small-inducible cytokine A2
Gene Aliases: Ccl2; Je; MCP-1; Mcp1; Scya2; Sigje
UniProt ID: (Rat) P14844
Entrez Gene ID: (Rat) 24770
MAPK cascade
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
positive regulation of endothelial cell proliferation
leukocyte migration involved in inflammatory response
monocyte chemotaxis
positive regulation of leukocyte migration
positive regulation of cellular extravasation
cellular calcium ion homeostasis
inflammatory response
cytoskeleton organization
transforming growth factor beta receptor signaling pathway
aging
glial cell migration
regulation of cell shape
response to heat
response to wounding
response to mechanical stimulus
response to bacterium
response to gamma radiation
regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
response to activity
negative regulation of angiogenesis
cytokine-mediated signaling pathway
positive regulation of cell-cell adhesion
neutrophil chemotaxis
organ regeneration
lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
response to progesterone
positive regulation of tumor necrosis factor production
cellular response to insulin stimulus
positive regulation of collagen biosynthetic process
response to vitamin B3
negative regulation of glial cell apoptotic process
helper T cell extravasation
cellular response to drug
cellular response to macrophage colony-stimulating factor stimulus
cellular response to platelet-derived growth factor stimulus
chemokinesis
response to drug
response to amino acid
protein kinase B signaling
negative regulation of neuron apoptotic process
astrocyte cell migration
cellular response to fibroblast growth factor stimulus
response to ethanol
positive regulation of cell adhesion
response to antibiotic
vascular endothelial growth factor receptor signaling pathway
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of synaptic transmission
positive regulation of T cell activation
response to glucocorticoid
positive regulation of nitric-oxide synthase biosynthetic process
maternal process involved in female pregnancy
maternal process involved in parturition
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to retinoic acid
cellular response to ATP
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to lipoprotein particle stimulus
cellular response to high density lipoprotein particle stimulus
cellular response to organic cyclic compound
cellular response to dexamethasone stimulus
positive regulation of monocyte chemotaxis
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of calcium ion import
positive regulation of protein targeting to membrane
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support