Invitrogen

MCP-1 Monoclonal Antibody (2D8)

17 Published Figures

26 References

View all (67) CCL2 (MCP-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (17)

MCP-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

MCP-1 Antibody (MA5-17040) in ICC/IF

Immunofluorescence analysis of HepG2 cells using CCL2 monoclonal antibody (Product # MA5-17040) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

MCP-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MCP-1 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-17040

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 4 publications 4 publications

Flow Cytometry (Flow)

1:200-1:400

View 2 publications 2 publications

ELISA (ELISA)

1:10,000

View 1 publication 1 publication

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2D8

Immunogen

Purified recombinant fragment of human CCL2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538512

Product Specific Information

MA5-17040 targets CCL2 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.

The MA5-17040 immunogen is purified recombinant fragment of human CCL2 expressed in E. Coli.

MA5-17040 detects CCL2 which has a predicted molecular weight of approximately 11kDa.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Four-and-a-Half LIM-Domain Protein 2 (FHL2) Induces Neuropeptide Y (NPY) in Macrophages in Visceral Adipose Tissue and Promotes Diet-Induced Obesity.

International journal of molecular sciences

Sommer J,Ehnis H,Seitz T,Schneider J,Wild AB,Moceri S,Buechler C,Bozec A,Weber GF,Merkel S,Beckervordersandforth R,Steinkasserer A,Schüle R,Trebicka J,Hartmann A,Bosserhoff A,von Hörsten S,Dietrich P,Hellerbrand C

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Immunohistochemistry (Paraffin)

Fri Oct 06 00:00:00 EDT 2023

Noncanonical atherosclerosis as the driving force in tricuspid aortic valve associated aneurysms - A trace collection.

Journal of lipid research

Doppler C,Messner B,Mimler T,Schachner B,Rezk M,Ganhör C,Wechselberger C,Müller M,Puh S,Pröll J,Arbeithuber B,Müller T,Zierer A,Bernhard D

MA5-17040 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that intimal atherosclerotic changes play a more central role in TAV-TAA formation than previously thought, particularly as the observed alterations do not follow classical patterns.

Wed Mar 01 00:00:00 EST 2023

Mechanical injury accentuates lipid deposition in ApoE^-/- mice and advance aortic valve stenosis: A novel modified aortic valve stenosis model.

Frontiers in cardiovascular medicine

Wen D,Hu L,Shan J,Zhang H,Hu L,Yuan A,Pu J,Xue S

MA5-17040 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show thickening and fibrosis of aortic valve leaflets in the hyperlipidemia group after wire injury.

Fri Feb 24 00:00:00 EST 2023

Environment-Sensitive Ectodomain Shedding of Epithin/PRSS14 Increases Metastatic Potential of Breast Cancer Cells by Producing CCL2.

Molecules and cells

Jang J,Cho EH,Cho Y,Ganzorig B,Kim KY,Kim MG,Kim C

MA5-17040 was used in Western Blotting to propose that epithin/PRSS14 can act as an environment-sensing receptor that promotes cancer metastasis by liberating the intracellular domain bearing transcriptional activity under conditions promoting ectodomain shedding.

Wed Aug 31 00:00:00 EDT 2022

Environment-Sensitive Ectodomain Shedding of Epithin/PRSS14 Increases Metastatic Potential of Breast Cancer Cells by Producing CCL2.

Molecules and cells

Jang J,Cho EH,Cho Y,Ganzorig B,Kim KY,Kim MG,Kim C

Wed Aug 31 00:00:00 EDT 2022

Mesenchymal stem cells alleviate systemic sclerosis by inhibiting the recruitment of pathogenic macrophages.

Cell death discovery

Gong P,Ding Y,Sun R,Jiang Z,Li W,Su X,Tian R,Zhou Y,Wang T,Jiang J,Li P,Shao C,Shi Y

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Immunocytochemistry

Sat Nov 26 00:00:00 EST 2022

Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes.

Scientific reports

Juin SK,Pushpakumar S,Tyagi SC,Sen U

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Western Blot

Thu Dec 15 00:00:00 EST 2022

Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes.

Scientific reports

Juin SK,Pushpakumar S,Tyagi SC,Sen U

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Western Blot

Thu Dec 15 00:00:00 EST 2022

Epstein-Barr viral product-containing exosomes promote fibrosis and nasopharyngeal carcinoma progression through activation of YAP1/FAPα signaling in fibroblasts.

Journal of experimental & clinical cancer research : CR

Lee PJ,Sui YH,Liu TT,Tsang NM,Huang CH,Lin TY,Chang KP,Liu SC

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Neutralization

Sat Aug 20 00:00:00 EDT 2022

Activity of acute pancreatitis is modified by secreted protein acidic and rich in cysteine ablation.

United European gastroenterology journal

Ammer-Herrmenau C,Wolf L,Nasrin SS,Ramu I,Roggiolani R,Goetze RG,Buchholz SM,Sendler M,Ellenrieder V,Neesse A

MA5-17040 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the role of secreted protein acidic and rich in cysteine (SPARC) during cerulein-induced AP in mice.

Fri Jul 01 00:00:00 EDT 2022

Epicardial placement of human MSC-loaded fibrin sealant films for heart failure: Preclinical efficacy and mechanistic data.

Molecular therapy : the journal of the American Society of Gene Therapy

Fields L,Ito T,Kobayashi K,Ichihara Y,Podaru MN,Hussain M,Yamashita K,Machado V,Lewis-McDougall F,Suzuki K

MA5-17040 was used in ELISA, Flow Cytometry to study the efficacy of mesenchymal stromal cell (MSC)-dressings using human amniotic membrane-derived MSCs and the underpinning mechanism for myocardial repair.

Wed Aug 04 00:00:00 EDT 2021

Epicardial placement of human MSC-loaded fibrin sealant films for heart failure: Preclinical efficacy and mechanistic data.

Molecular therapy : the journal of the American Society of Gene Therapy

Fields L,Ito T,Kobayashi K,Ichihara Y,Podaru MN,Hussain M,Yamashita K,Machado V,Lewis-McDougall F,Suzuki K

Wed Aug 04 00:00:00 EDT 2021

Epicardial placement of human MSC-loaded fibrin sealant films for heart failure: Preclinical efficacy and mechanistic data.

Molecular therapy : the journal of the American Society of Gene Therapy

Fields L,Ito T,Kobayashi K,Ichihara Y,Podaru MN,Hussain M,Yamashita K,Machado V,Lewis-McDougall F,Suzuki K

Wed Aug 04 00:00:00 EDT 2021

Tart Cherry Juice and Seeds Affect Pro-Inflammatory Markers in Visceral Adipose Tissue of High-Fat Diet Obese Rats.

Molecules (Basel, Switzerland)

Moruzzi M,Klöting N,Blüher M,Martinelli I,Tayebati SK,Gabrielli MG,Roy P,Micioni Di Bonaventura MV,Cifani C,Lupidi G,Amenta F,Tomassoni D

MA5-17040 was used in Immunohistochemistry to investigate the potential positive effects of tart cherries on rats with Diet-Induced Obesity (DIO).

Fri Mar 05 00:00:00 EST 2021

Tart Cherry Juice and Seeds Affect Pro-Inflammatory Markers in Visceral Adipose Tissue of High-Fat Diet Obese Rats.

Molecules (Basel, Switzerland)

Moruzzi M,Klöting N,Blüher M,Martinelli I,Tayebati SK,Gabrielli MG,Roy P,Micioni Di Bonaventura MV,Cifani C,Lupidi G,Amenta F,Tomassoni D

MA5-17040 was used in Immunohistochemistry to investigate the potential positive effects of tart cherries on rats with Diet-Induced Obesity (DIO).

Fri Mar 05 00:00:00 EST 2021

Tart Cherry Juice and Seeds Affect Pro-Inflammatory Markers in Visceral Adipose Tissue of High-Fat Diet Obese Rats.

Molecules (Basel, Switzerland)

Moruzzi M,Klöting N,Blüher M,Martinelli I,Tayebati SK,Gabrielli MG,Roy P,Micioni Di Bonaventura MV,Cifani C,Lupidi G,Amenta F,Tomassoni D

MA5-17040 was used in Immunohistochemistry to investigate the potential positive effects of tart cherries on rats with Diet-Induced Obesity (DIO).

Fri Mar 05 00:00:00 EST 2021

IL-15 Prevents Renal Fibrosis by Inhibiting Collagen Synthesis: A New Pathway in Chronic Kidney Disease?

International journal of molecular sciences

Devocelle A,Lecru L,Ferlicot S,Bessede T,Candelier JJ,Giron-Michel J,François H

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Flow Cytometry

Thu Oct 28 00:00:00 EDT 2021

Impact of the rs1024611 Polymorphism of CCL2 on the Pathophysiology and Outcome of Primary Myelofibrosis.

Cancers

Masselli E,Carubbi C,Pozzi G,Percesepe A,Campanelli R,Villani L,Gobbi G,Bonomini S,Roti G,Rosti V,Massa M,Barosi G,Vitale M

MA5-17040 was used in Western Blot to conclude that the identification of the role of the CCL2/CCR2 chemokine system in PMF adds a novel element to the pathophysiological picture of the disease, with clinical and therapeutic implications.

Sat May 22 00:00:00 EDT 2021

Impact of the rs1024611 Polymorphism of CCL2 on the Pathophysiology and Outcome of Primary Myelofibrosis.

Cancers

Masselli E,Carubbi C,Pozzi G,Percesepe A,Campanelli R,Villani L,Gobbi G,Bonomini S,Roti G,Rosti V,Massa M,Barosi G,Vitale M

Sat May 22 00:00:00 EDT 2021

Assessment of Immunological Potential of Glial Restricted Progenitor Graft In Vivo-Is Immunosuppression Mandatory?

Cells

Kozlowska U,Klimczak A,Bednarowicz KA,Zalewski T,Rozwadowska N,Chojnacka K,Jurga S,Barnea ER,Kurpisz MK

MA5-17040 was used in Immunohistochemistry and Immunocytochemistry to research the efficiency of glial restricted progenitors grafted into murine CNS in healthy models and disease models.

Fri Jul 16 00:00:00 EDT 2021

Assessment of Immunological Potential of Glial Restricted Progenitor Graft In Vivo-Is Immunosuppression Mandatory?

Cells

Kozlowska U,Klimczak A,Bednarowicz KA,Zalewski T,Rozwadowska N,Chojnacka K,Jurga S,Barnea ER,Kurpisz MK

MA5-17040 was used in Immunohistochemistry and Immunocytochemistry to research the efficiency of glial restricted progenitors grafted into murine CNS in healthy models and disease models.

Fri Jul 16 00:00:00 EDT 2021

Wnt5a promotes renal tubular inflammation in diabetic nephropathy by binding to CD146 through noncanonical Wnt signaling.

Cell death & disease

Li X,Wen J,Dong Y,Zhang Q,Guan J,Liu F,Zhou T,Li Z,Fan Y,Wang N

MA5-17040 was used in Immunohistochemistry-immunofluorescence to suggest that by directly binding to CD146, Wnt5a-induced noncanonical signaling is a contributing mechanism for renal tubular inflammation in diabetic nephropathy.

Mon Jan 18 00:00:00 EST 2021

Wnt5a promotes renal tubular inflammation in diabetic nephropathy by binding to CD146 through noncanonical Wnt signaling.

Cell death & disease

Li X,Wen J,Dong Y,Zhang Q,Guan J,Liu F,Zhou T,Li Z,Fan Y,Wang N

Mon Jan 18 00:00:00 EST 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

MA5-17040 was used in Western Blot to demonstrate that the reduced dimeric form of Gal-1 is responsible for inducing improvements to muscle membrane repair, while the monomeric form is responsible for changes in inflammation.

Wed Nov 17 00:00:00 EST 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

Wed Nov 17 00:00:00 EST 2021

Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Cancers

Gelsomino L,Naimo GD,Malivindi R,Augimeri G,Panza S,Giordano C,Barone I,Bonofiglio D,Mauro L,Catalano S,Andò S

MA5-17040 was used in Immunocytochemistry-immunoflourescence to evaluate how the tumour cell-macrophage interaction and breast cancer phenotype are affected by leptin receptor knockdown.

Mon Jul 27 00:00:00 EDT 2020

Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.

Nature

Tavora B,Mederer T,Wessel KJ,Ruffing S,Sadjadi M,Missmahl M,Ostendorf BN,Liu X,Kim JY,Olsen O,Welm AL,Goodarzi H,Tavazoie SF

MA5-17040 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate SLIT2 and ROBO1 in blood vessel formation.

Thu Oct 01 00:00:00 EDT 2020

The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.

Cancer cell

Su W,Han HH,Wang Y,Zhang B,Zhou B,Cheng Y,Rumandla A,Gurrapu S,Chakraborty G,Su J,Yang G,Liang X,Wang G,Rosen N,Scher HI,Ouerfelli O,Giancotti FG

MA5-17040 was used in Immunohistochemistry-immunofluorescence to reveal that PRC1 coordinates stemness with immune evasion and neoangiogenesis and point to the potential clinical utility of targeting PRC1 in DNPC.

Mon Aug 12 00:00:00 EDT 2019

Liver macrophage-associated inflammation correlates with SIV burden and is substantially reduced following cART.

PLoS pathogens

Fisher BS,Green RR,Brown RR,Wood MP,Hensley-McBain T,Fisher C,Chang J,Miller AD,Bosche WJ,Lifson JD,Mavigner M,Miller CJ,Gale M,Silvestri G,Chahroudi A,Klatt NR,Sodora DL

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Immunohistochemistry

Thu Feb 01 00:00:00 EST 2018

Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress.

Redox biology

Li DJ,Fu H,Tong J,Li YH,Qu LF,Wang P,Shen FM

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Immunohistochemistry

Tue May 01 00:00:00 EDT 2018

Neutrophil Microvesicles from Healthy Control and Rheumatoid Arthritis Patients Prevent the Inflammatory Activation of Macrophages.

EBioMedicine

Rhys HI,Dell'Accio F,Pitzalis C,Moore A,Norling LV,Perretti M

Published figure using MCP-1 monoclonal antibody (Product # MA5-17040) in Immunofluorescence

Thu Mar 01 00:00:00 EST 2018

Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B

MA517040 was used in western blot to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis

Wed Nov 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2

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Gene Aliases: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Mouse) 20296

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Function(s)

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding G-protein coupled receptor binding cytokine activity protein binding cytokine intercellular signal molecule

Process(es)

Performance Guarantee

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MCP-1 Monoclonal Antibody (2D8)

MCP-1 Antibody (MA5-17040) in ICC/IF

Product Details

MA5-17040

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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