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MCP-2 Antibody (500-M69-500UG) in ELISA
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of MCP-2 Monoclonal Antibody (Product # 500-M69-500UG) will detect at least 500 pg/mL of Recombinant Human MCP-2 (CCL8) when used with PeproTech MCP-2 Polyclonal Antibody, Biotin (Product # 500-P35BT-1MG) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL.
Product Details
500-M69-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MCP-2 (CCL8)
Conjugate
Form
Lyophilized
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2942205
Product Specific Information
AA Sequence of recombinant protein: QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP.
Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-2 (CCL8). Anti-Human MCP-2 (CCL8)-specific antibody was purified from cell culture by Protein G affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human MCP-2 (CCL8) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-2 (CCL8) (500-P35BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL.
Western Blot: To detect Human MCP-2 (CCL8) by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-2 (CCL8) is 0.25-0.50 ng/lane, under non-reducing conditions.
Target Information
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP-2; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); Small-inducible cytokine A8
Gene Aliases: CCL8; HC14; MCP-2; MCP2; SCYA10; SCYA8
UniProt ID: (Human) P80075
Entrez Gene ID: (Human) 6355
monocyte chemotaxis
positive regulation of leukocyte migration
protein phosphorylation
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to virus
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
negative regulation of leukocyte proliferation
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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