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MCP-2 Antibody (500-P127BT-25UG) in ELISA
Sandwich ELISA: To detect Murine MCP-2 (CCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MCP-2 Polyclonal Antibody, Biotin (Product # 500-P127BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-2 Polyclonal Antibody (Product # 500-P127-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-2 (CCL8).
Product Details
500-P127BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MCP-2 (CCL8)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929370
Product Specific Information
AA Sequence of recombinant protein: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-2 (CCL8). Anti-Murine MCP-2 (CCL8)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine MCP-2 (CCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-2 (CCL8) (500-P127) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-2 (CCL8).
Western Blot: To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P127BT-1MG will be provided as 2 x 500 µg
Target Information
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 8; MCP-2; Monocyte chemoattractant protein 2; monocyte chemoattractant protein-2; Monocyte chemotactic protein 2; small inducible cytokine A8; Small-inducible cytokine A8
Gene Aliases: 1810063B20Rik; AB023418; Ccl8; HC14; MCP-2; Mcp2; Scya8
UniProt ID: (Mouse) Q9Z121
Entrez Gene ID: (Mouse) 20307
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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