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MCP-2 Antibody (500-P35BT-25UG) in ELISA
Sandwich ELISA: To detect hMCP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MCP-2 Polyclonal Antibody, Biotin (Product # 500-P35BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-2 Polyclonal Antibody (Product # 500-P35-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMCP-2.
Product Details
500-P35BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MCP-2 (CCL8)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930483
Product Specific Information
AA Sequence of recombinant protein: QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-2 (CCL8). Anti-Human MCP-2 (CCL8)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMCP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MCP-2 (500-P35) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMCP-2.
Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P35BT-1MG will be provided as 2 x 500 µg
Target Information
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP-2; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); Small-inducible cytokine A8
Gene Aliases: CCL8; HC14; MCP-2; MCP2; SCYA10; SCYA8
UniProt ID: (Human) P80075
Entrez Gene ID: (Human) 6355
monocyte chemotaxis
positive regulation of leukocyte migration
protein phosphorylation
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to virus
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
negative regulation of leukocyte proliferation
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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