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MCP-3 Antibody (500-M73-500UG) in WB
Western Blot: To detect Human MCP-3 (CCL7) by Western Blot analysis MCP-3 Monoclonal Antibody (Product # 500-M73-500UG) can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 (CCL7) is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Product Details
500-M73-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MCP-3 (CCL7)
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929126
Product Specific Information
AA Sequence of recombinant protein: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL.
Preparation: Produced in C3H/HeJ mice immunized with highly pure Recombinant Human MCP-3 (CCL7). Anti-Human MCP-3 (CCL7)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect Recombinant Human MCP-3 (CCL7) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-3 (CCL7) (500-P37BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.
Western Blot: To detect Human MCP-3 (CCL7) by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 (CCL7) is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Target Information
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 7; chemokine (C-C motif) ligand 7; H-MCP-3; MCP-3; MGC138463; MGC138465; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; small inducible cytokine A7 (monocyte chemotactic protein 3); Small-inducible cytokine A7
Gene Aliases: CCL7; FIC; MARC; MCP-3; MCP3; NC28; SCYA6; SCYA7
UniProt ID: (Human) P80098
Entrez Gene ID: (Human) 6354
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
response to gamma radiation
positive regulation of cell migration
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to ethanol
positive regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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