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MCP-3 Antibody (500-P116GBT-25UG) in ELISA
Sandwich ELISA: To detect Murine MCP-3 (CCL7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MCP-3 Polyclonal Antibody, Biotin (Product # 500-P116GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-3 Polyclonal Antibody (Product # 500-P116G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-3 (CCL7).
Product Details
500-P116GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MCP-3 (CCL7)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929292
Product Specific Information
AA Sequence of recombinant protein: QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine MCP-3 (CCL7). Anti-Murine MCP-3 (CCL7)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine MCP-3 (CCL7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-3 (CCL7) (500-P116G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MCP-3 (CCL7).
Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P116GBT-1MG will be provided as 2 x 500 µg
Target Information
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 7; H-MCP-3; Intercrine/chemokine MARC; MCP-3; MGC138463; MGC138465; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; Protein FIC; RANTES/sis homolog; RP23-350G1.4; small inducible cytokine A7; Small-inducible cytokine A7
Gene Aliases: Ccl7; Fic; marc; MCP-3; Mcp3; Scya7
UniProt ID: (Mouse) Q03366
Entrez Gene ID: (Mouse) 20306
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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