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MCP-4 Antibody (500-M70-500UG) in ELISA
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of MCP-4 Monoclonal Antibody (Product # 500-M70-500UG) will detect at least 100 pg/mL of Recombinant Human MCP-4 (CCL13) when used with PeproTech MCP-4 Polyclonal Antibody, Biotin (Product # 500-P04GBT-1MG) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.
Product Details
500-M70-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MCP-4 (CCL13)
Conjugate
Form
Lyophilized
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2942206
Product Specific Information
AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT.
Preparation: Produced in mice immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified from cell culture by Protein G affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-4 (CCL13) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-4 (CCL13) (500-P04GBT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.
Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Target Information
Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 13; CCL 13; chemokine (C-C motif) ligand 13; CK-beta-10; H-MCP-4; MCP-4; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC-1; new CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 13; Small-inducible cytokine A13
Gene Aliases: CCL13; CKb10; MCP-4; MCP4; NCC-1; NCC1; SCYA13; SCYL1
UniProt ID: (Human) Q99616
Entrez Gene ID: (Human) 6357
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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