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MCP-4 Antibody (500-P04-1MG) in WB
Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis MCP-4 Polyclonal Antibody (Product # 500-P04-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P04-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MCP-4 (CCL13)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929148
Product Specific Information
AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-4 matrix.
Sandwich ELISA: To detect Human MCP-4 (CCL13) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-4 (CCL13) (500-P04BT) as a detection antibody, allows the detection of at least 2000- 4000 pg/mL of Recombinant Human MCP-4 (CCL13).
Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 13; CCL 13; chemokine (C-C motif) ligand 13; CK-beta-10; H-MCP-4; MCP-4; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC-1; new CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 13; Small-inducible cytokine A13
Gene Aliases: CCL13; CKb10; MCP-4; MCP4; NCC-1; NCC1; SCYA13; SCYL1
UniProt ID: (Human) Q99616
Entrez Gene ID: (Human) 6357
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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