Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
MCP-4 Antibody (M810) in ELISA
Sandwich ELISA analysis of an anti-human MCP-4 monoclonal antibody (Product # M810) was performed by loading 100 µL per well of antibody (Product # M810) at a concentration of 2 µg/mL overnight at room temperature. The plate was washed 3 times with ELISA Wash Buffer (Product # N503), and 100 µL of recombinant human MCP-4/CCL13 was added to wells in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0pg/mL concentrations and the samples were incubated for 2 hours at room temperature. The plate was washed, then incubated with 100 µL pe... View More
Product Details
M810
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8C12
Immunogen
Recombinant CCL13 protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536662
Product Specific Information
The M810 MCP-4 antibody (clone 8C12) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M809 (biotinylated conjugate of clone 3G4). The M810 MCP-4 antibody (biotinylated conjugate of clone 8C12) has also been used successfully in a sandwich ELISA as a detection antibody when paired with M809 (clone 3G4) as the coating antibody.
Target Information
Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C-C motif chemokine 13; CCL 13; chemokine (C-C motif) ligand 13; CK-beta-10; H-MCP-4; MCP-4; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC-1; new CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 13; Small-inducible cytokine A13
Gene Aliases: CCL13; CKb10; MCP-4; MCP4; NCC-1; NCC1; SCYA13; SCYL1
UniProt ID: (Human) Q99616
Entrez Gene ID: (Human) 6357
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support