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MCP-5 Antibody (500-P61BT-25UG) in ELISA
Sandwich ELISA: To detect mMCP-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MCP-5 Polyclonal Antibody, Biotin (Product # 500-P61BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-5 Polyclonal Antibody (Product # 500-P61-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMCP-5.
Product Details
500-P61BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MCP-5 (CCL12)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930657
Product Specific Information
AA Sequence of recombinant protein: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MCP-5 (CCL12). Anti-Murine MCP-5 (CCL12)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mMCP-5 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MCP-5 (500-P61) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMCP-5.
Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P61BT-1MG will be provided as 2 x 500 µg
Target Information
Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 12; MCP-1-related chemokine; MCP-5; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; small inducible cytokine A12; Small-inducible cytokine A12
Gene Aliases: Ccl12; MCP-5; Mcp5; Scya12
UniProt ID: (Mouse) Q62401
Entrez Gene ID: (Mouse) 20293
MAPK cascade
angiogenesis
monocyte chemotaxis
positive regulation of leukocyte migration
chemotaxis
inflammatory response
immune response
cytoskeleton organization
regulation of cell shape
cytokine-mediated signaling pathway
lipopolysaccharide-mediated signaling pathway
negative regulation of glial cell apoptotic process
protein kinase B signaling
negative regulation of neuron apoptotic process
astrocyte cell migration
macrophage chemotaxis
negative regulation of leukocyte proliferation
cellular response to organic cyclic compound
positive regulation of calcium ion import
negative regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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