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MDC Antibody (500-P176BT-25UG) in ELISA
Sandwich ELISA: To detect mMDC by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MDC Polyclonal Antibody, Biotin (Product # 500-P176BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MDC Polyclonal Antibody (Product # 500-P176-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMDC.
Product Details
500-P176BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MDC (CCL22)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929652
Product Specific Information
AA Sequence of recombinant protein: GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MDC (CCL22). Anti-Murine MDC (CCL22)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mMDC by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MDC (500-P176) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mMDC.
Western Blot: To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mMDC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P176BT-1MG will be provided as 2 x 500 µg
Target Information
CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Also, it is a chemotactic for monocytes. dendritic cells and natural killer cells. CCL22 is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activated B and dendritic cell-derived; AW060611; C-C motif chemokine 22; CC chemokine ABCD-1; dendritic cell and B cell derived chemokine; MGC108943; MGC34554; RP24-69A20.1; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED); small inducible cytokine subfamily A, member 22; small inducible cytokine subfamily A22; Small-inducible cytokine A22
Gene Aliases: ABCD-1; Abcd1; Ccl22; DCBCK; MDC; Scya22
UniProt ID: (Mouse) O88430
Entrez Gene ID: (Mouse) 20299
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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