Proteintech

ME1 Polyclonal Antibody

ME1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ME1 Antibody (16619-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).

ME1 Antibody in Immunoprecipitation (IP)

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Product Details

16619-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ME1 Fusion Protein Ag9916 (226-572 aa encoded by BC025246)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DEFMEAVSS KYGMNCLIQF EDFANVNAFR LLNKYRNQYC TFNDDIQGTA SVAVAGLLAA LRITKNKLSD QTILFQGAGE AALGIAHLIV MALEKEGLPK EKAIKKIWLV DSKGLIVKGR ASLTQEKEKF AHEHEEMKNL EAIVQEIKPT ALIGVAAIGG AFSEQILKDM AAFNERPIIF ALSNPTSKAE CSAEQCYKIT KGRAIFASGS PFDPVTLPNG QTLYPGQGNN SYVFPGVALG VVACGLRQIT DNIFLTTAEV IAQQVSDKHL EEGRLYPPLN TIRDVSLKIA EKIVKDAYQE KTATVYPEPQ NKEAFVRSQM YSTDYDQILP DCYSWPEEVQ KIQTKVDQ (226-572 aa encoded by BC025246)

Target Information

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic analysis identifies Stomatin as a biological marker for psychological stress.

Neurobiology of stress

Cao Y,Ying SQ,Qiu XY,Guo J,Chen C,Li SJ,Dou G,Zheng CX,Chen D,Qiu JY,Jin Y,Sui BD,Jin F

Sun Jan 01 00:00:00 EST 2023

Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Fang X,Zhang J,Li Y,Song Y,Yu Y,Cai Z,Lian F,Yang J,Min J,Wang F

Mon May 01 00:00:00 EDT 2023

Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Fang X,Zhang J,Li Y,Song Y,Yu Y,Cai Z,Lian F,Yang J,Min J,Wang F

Mon May 01 00:00:00 EDT 2023

Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics.

Nature metabolism

Liu Q,Zhu F,Liu X,Lu Y,Yao K,Tian N,Tong L,Figge DA,Wang X,Han Y,Li Y,Zhu Y,Hu L,Ji Y,Xu N,Li D,Gu X,Liang R,Gan G,Wu L,Zhang P,Xu T,Hu H,Hu Z,Xu H,Ye D,Yang H,Li B,Tong X

Sun May 01 00:00:00 EDT 2022

USP19 exacerbates lipogenesis and colorectal carcinogenesis by stabilizing ME1.

Cell reports

Zhu Y,Gu L,Lin X,Zhou X,Lu B,Liu C,Lei C,Zhou F,Zhao Q,Prochownik EV,Li Y

Tue Dec 28 00:00:00 EST 2021

miR-885-5p Inhibits Invasion and Metastasis in Gastric Cancer by Targeting Malic Enzyme 1.

DNA and cell biology

Jiang Z,Cui H,Zeng S,Li L

Sat May 01 00:00:00 EDT 2021

Short hairpin RNA attenuates liver fibrosis by regulating the PPAR‑γ and NF‑κB pathways in HBV‑induced liver fibrosis in mice.

International journal of oncology

Ye L,Chen T,Cao J,Sun L,Li W,Zhang C

Sun Nov 01 00:00:00 EDT 2020

Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression.

Oxidative medicine and cellular longevity

Qi J,Chen X,Wu Q,Wang J,Zhang H,Mao A,Zhu M,Miao C

Tue Nov 17 00:00:00 EST 2020

Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yu HF,Duan CC,Yang ZQ,Wang YS,Yue ZP,Guo B

Sun Mar 01 00:00:00 EST 2020

Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yu HF,Duan CC,Yang ZQ,Wang YS,Yue ZP,Guo B

Sun Mar 01 00:00:00 EST 2020

Dissecting cell-type-specific metabolism in pancreatic ductal adenocarcinoma.

eLife

Lau AN,Li Z,Danai LV,Westermark AM,Darnell AM,Ferreira R,Gocheva V,Sivanand S,Lien EC,Sapp KM,Mayers JR,Biffi G,Chin CR,Davidson SM,Tuveson DA,Jacks T,Matheson NJ,Yilmaz O,Vander Heiden MG

Fri Jul 10 00:00:00 EDT 2020

Dissecting cell-type-specific metabolism in pancreatic ductal adenocarcinoma.

eLife

Lau AN,Li Z,Danai LV,Westermark AM,Darnell AM,Ferreira R,Gocheva V,Sivanand S,Lien EC,Sapp KM,Mayers JR,Biffi G,Chin CR,Davidson SM,Tuveson DA,Jacks T,Matheson NJ,Yilmaz O,Vander Heiden MG

Fri Jul 10 00:00:00 EDT 2020

Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis.

Molecular cell

Zhu Y,Gu L,Lin X,Liu C,Lu B,Cui K,Zhou F,Zhao Q,Prochownik EV,Fan C,Li Y

Thu Jan 02 00:00:00 EST 2020

Malic Enzyme 1 (ME1) is pro-oncogenic in Apc^Min/+ mice.

Scientific reports

Fernandes LM,Al-Dwairi A,Simmen RCM,Marji M,Brown DM,Jewell SW,Simmen FA

Mon Sep 24 00:00:00 EDT 2018

Malic Enzyme 1 (ME1) is pro-oncogenic in Apc^Min/+ mice.

Scientific reports

Fernandes LM,Al-Dwairi A,Simmen RCM,Marji M,Brown DM,Jewell SW,Simmen FA

Mon Sep 24 00:00:00 EDT 2018

Malic Enzyme 1 (ME1) is pro-oncogenic in Apc^Min/+ mice.

Scientific reports

Fernandes LM,Al-Dwairi A,Simmen RCM,Marji M,Brown DM,Jewell SW,Simmen FA

Mon Sep 24 00:00:00 EDT 2018

Malic Enzyme 1 (ME1) is pro-oncogenic in Apc^Min/+ mice.

Scientific reports

Fernandes LM,Al-Dwairi A,Simmen RCM,Marji M,Brown DM,Jewell SW,Simmen FA

Mon Sep 24 00:00:00 EDT 2018

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 EST 2017

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Oncotarget

Gu Z,Zhang H,Li Y,Shen S,Yin X,Zhang W,Cheng R,Zhang Y,Zhang X,Chen H,Huang B,Cao Y

Tue Dec 26 00:00:00 EST 2017

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice.

PloS one

Al-Dwairi A,Brown AR,Pabona JM,Van TH,Hamdan H,Mercado CP,Quick CM,Wight PA,Simmen RC,Simmen FA

Tue Jan 19 00:00:00 EST 2016

Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice.

PloS one

Al-Dwairi A,Brown AR,Pabona JM,Van TH,Hamdan H,Mercado CP,Quick CM,Wight PA,Simmen RC,Simmen FA

Tue Jan 19 00:00:00 EST 2016

Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice.

PloS one

Al-Dwairi A,Brown AR,Pabona JM,Van TH,Hamdan H,Mercado CP,Quick CM,Wight PA,Simmen RC,Simmen FA

Tue Jan 19 00:00:00 EST 2016

Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice.

PloS one

Al-Dwairi A,Brown AR,Pabona JM,Van TH,Hamdan H,Mercado CP,Quick CM,Wight PA,Simmen RC,Simmen FA

Tue Jan 19 00:00:00 EST 2016

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Cancer cell

Mitsuishi Y,Taguchi K,Kawatani Y,Shibata T,Nukiwa T,Aburatani H,Yamamoto M,Motohashi H

Tue Jul 10 00:00:00 EDT 2012

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Cancer cell

Mitsuishi Y,Taguchi K,Kawatani Y,Shibata T,Nukiwa T,Aburatani H,Yamamoto M,Motohashi H

Tue Jul 10 00:00:00 EDT 2012

Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, endocrine profile, and gastrointestinal tract proliferation-associated biomarkers in male mice.

PloS one

Al-Dwairi A,Pabona JM,Simmen RC,Simmen FA

Fri May 03 00:00:00 EDT 2013

Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, endocrine profile, and gastrointestinal tract proliferation-associated biomarkers in male mice.

PloS one

Al-Dwairi A,Pabona JM,Simmen RC,Simmen FA

Fri May 03 00:00:00 EDT 2013

Proteomic profiling of adipose tissue from Zmpste24-/- mice, a model of lipodystrophy and premature aging, reveals major changes in mitochondrial function and vimentin processing.

Molecular & cellular proteomics : MCP

Peinado JR,Quirós PM,Pulido MR,Mariño G,Martínez-Chantar ML,Vázquez-Martínez R,Freije JM,López-Otín C,Malagón MM

Tue Nov 01 00:00:00 EDT 2011

Proteomic profiling of adipose tissue from Zmpste24-/- mice, a model of lipodystrophy and premature aging, reveals major changes in mitochondrial function and vimentin processing.

Molecular & cellular proteomics : MCP

Peinado JR,Quirós PM,Pulido MR,Mariño G,Martínez-Chantar ML,Vázquez-Martínez R,Freije JM,López-Otín C,Malagón MM

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: cytosolic malic enzyme 1; malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); Malic enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; malic enzyme, supernatant; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase

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Gene Aliases: D9Ertd267e; HUMNDME; Mdh-1; ME1; MES; Mod-1; MOD1

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UniProt ID: (Human) P48163, (Mouse) P06801

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Entrez Gene ID: (Human) 4199, (Mouse) 17436, (Rat) 24552

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Function(s)

malic enzyme activity malate dehydrogenase (decarboxylating) (NAD+) activity malate dehydrogenase (decarboxylating) (NADP+) activity oxaloacetate decarboxylase activity electron carrier activity manganese ion binding ADP binding NADP binding NAD binding protein binding oxidoreductase activity metal ion binding oxidoreductase

Process(es)

carbohydrate metabolic process malate metabolic process NADP biosynthetic process response to hormone response to carbohydrate protein tetramerization oxidation-reduction process regulation of NADP metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ME1 Polyclonal Antibody

ME1 Antibody (16619-1-AP) in IHC (P)

Product Details

16619-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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