Proteintech

MECP2 Polyclonal Antibody

MECP2 Antibody in Immunocytochemistry (ICC/IF)

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MECP2 Antibody (10861-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10861-1-AP (MECP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MECP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MECP2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10861-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MECP2 Fusion Protein Ag1309 (138-487 aa encoded by BC011612)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa.

Immunogen sequence: LIAYFEKVG DTSLDPNDFD FTVTGRGSPS RREQKPPKKP KSPKAPGTGR GRGRPKGSGT TRPKAATSEG VQVKRVLEKS PGKLLVKMPF QTSPGGKAEG GGATTSTQVM VIKRPGRKRK AEADPQAIPK KRGRKPGSVV AAAAAEAKKK AVKESSIRSV QETVLPIKKR KTRETVSIEV KEVVKPLLVS TLGEKSGKGL KTCKSPGRKS KESSPKGRSS SASSPPKKEH HHHHHHSESP KAPVPLLPPL PPPPPEPESS EDPTSPPEPQ DLSSSVCKEE KMPRGGSLES DGCPKEPAKT QPAVATAATA AEKYKHRGEG ERKDIVSSSM PRPNREEPVD SRTPVTERVS (138-487 aa encoded by BC011612)

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of DNA methylation de-represses peroxisome proliferator-activated receptor-γ and attenuates pulmonary fibrosis.

British journal of pharmacology

Wei A,Gao Q,Chen F,Zhu X,Chen X,Zhang L,Su X,Dai J,Shi Y,Cao W

Fri Apr 01 00:00:00 EDT 2022

SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.

International journal of biological macromolecules

Wang R,Yang M,Wu Y,Liu R,Liu M,Li Q,Su X,Xin Y,Huo W,Deng Q,Ba Y,Huang H

Fri Sep 30 00:00:00 EDT 2022

A prelude to the proximity interaction mapping of CXXC5.

Scientific reports

Ayaz G,Turan G,Olgun ÇE,Kars G,Karakaya B,Yavuz K,Demiralay ÖD,Can T,Muyan M,Yaşar P

Thu Sep 02 00:00:00 EDT 2021

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Nature communications

Wang XH,Xu S,Zhou XY,Zhao R,Lin Y,Cao J,Zang WD,Tao H,Xu W,Li MQ,Zhao SM,Jin LP,Zhao JY

Tue Jun 08 00:00:00 EDT 2021

MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.

Science (New York, N.Y.)

Ibrahim A,Papin C,Mohideen-Abdul K,Le Gras S,Stoll I,Bronner C,Dimitrov S,Klaholz BP,Hamiche A

Fri Jun 25 00:00:00 EDT 2021

Integration of transcriptome and proteome profiles in placenta accreta reveals trophoblast over-migration as the underlying pathogenesis.

Clinical proteomics

Li N,Hou R,Liu C,Yang T,Qiao C,Wei J

Wed Dec 29 00:00:00 EST 2021

Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice.

Epigenetics & chromatin

Buck JM,O'Neill HC,Stitzel JA

Thu Mar 05 00:00:00 EST 2020

MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation.

International journal of medical sciences

Huang YH,Kuo HC,Yang YL,Wang FS

Wed Jun 05 00:00:00 EDT 2019

Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro.

Molecular medicine reports

Song N,Li K,Wang Y,Chen Z,Shi L

Fri Jan 01 00:00:00 EST 2016

Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro.

Molecular medicine reports

Song N,Li K,Wang Y,Chen Z,Shi L

Fri Jan 01 00:00:00 EST 2016

Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2.

Clinical immunology (Orlando, Fla.)

Basu A,Woods-Burnham L,Ortiz G,Rios-Colon L,Figueroa J,Albesa R,Andrade LE,Mahler M,Casiano CA

Tue Dec 01 00:00:00 EST 2015

Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2.

Clinical immunology (Orlando, Fla.)

Basu A,Woods-Burnham L,Ortiz G,Rios-Colon L,Figueroa J,Albesa R,Andrade LE,Mahler M,Casiano CA

Tue Dec 01 00:00:00 EST 2015

Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2.

Clinical immunology (Orlando, Fla.)

Basu A,Woods-Burnham L,Ortiz G,Rios-Colon L,Figueroa J,Albesa R,Andrade LE,Mahler M,Casiano CA

Tue Dec 01 00:00:00 EST 2015

Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2.

Clinical immunology (Orlando, Fla.)

Basu A,Woods-Burnham L,Ortiz G,Rios-Colon L,Figueroa J,Albesa R,Andrade LE,Mahler M,Casiano CA

Tue Dec 01 00:00:00 EST 2015

The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity.

Molecular cancer research : MCR

Leoh LS,van Heertum B,De Rijck J,Filippova M,Rios-Colon L,Basu A,Martinez SR,Tungteakkhun SS,Filippov V,Christ F,De Leon M,Debyser Z,Casiano CA

Thu Mar 01 00:00:00 EST 2012

The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity.

Molecular cancer research : MCR

Leoh LS,van Heertum B,De Rijck J,Filippova M,Rios-Colon L,Basu A,Martinez SR,Tungteakkhun SS,Filippov V,Christ F,De Leon M,Debyser Z,Casiano CA

Thu Mar 01 00:00:00 EST 2012

Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury.

Brain research

Wang Y,Liu C,Guo QL,Yan JQ,Zhu XY,Huang CS,Zou WY

Tue Oct 18 00:00:00 EDT 2011

Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury.

Brain research

Wang Y,Liu C,Guo QL,Yan JQ,Zhu XY,Huang CS,Zou WY

Tue Oct 18 00:00:00 EDT 2011

Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury.

Brain research

Wang Y,Liu C,Guo QL,Yan JQ,Zhu XY,Huang CS,Zou WY

Tue Oct 18 00:00:00 EDT 2011

Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury.

Brain research

Wang Y,Liu C,Guo QL,Yan JQ,Zhu XY,Huang CS,Zou WY

Tue Oct 18 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: DKFZp686A24160; MeCp-2 protein; methyl CpG binding protein 2; Methyl-CpG-binding protein 2; testis tissue sperm-binding protein Li 41a

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Gene Aliases: 1500041B07Rik; AUTSX3; D630021H01Rik; Mbd5; MECP2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP10

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UniProt ID: (Human) P51608, (Mouse) Q9Z2D6, (Rat) Q00566

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Entrez Gene ID: (Human) 4204, (Mouse) 17257, (Rat) 29386

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Function(s)

four-way junction DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity mRNA binding protein binding transcription factor binding methyl-CpG binding double-stranded methylated DNA binding protein domain specific binding chromatin DNA binding siRNA binding histone deacetylase binding poly(A) RNA binding unmethylated CpG binding protein N-terminus binding chromatin binding enzyme binding DNA metabolism protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter behavioral fear response response to hypoxia startle response neurological system process involved in regulation of systemic arterial blood pressure regulation of respiratory gaseous exchange by neurological system process inositol metabolic process mitochondrial electron transport, ubiquinol to cytochrome c chromatin silencing regulation of gene expression by genetic imprinting transcription, DNA-templated glutamine metabolic process cellular biogenic amine metabolic process synapse assembly heart development respiratory gaseous exchange long-term memory locomotory behavior protein localization glucocorticoid metabolic process positive regulation of cell proliferation adult locomotory behavior visual learning response to radiation pathogenesis post-embryonic development response to ionizing radiation response to lead ion dendrite development histone methylation histone acetylation proprioception sensory perception of pain cerebellum development ventricular system development negative regulation of histone methylation cardiolipin metabolic process response to estradiol negative regulation of histone acetylation social behavior neuron maturation negative regulation of neuron apoptotic process regulation of DNA methylation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated phosphatidylcholine metabolic process negative regulation of astrocyte differentiation catecholamine secretion positive regulation of synapse assembly excitatory postsynaptic potential long-term synaptic potentiation regulation of transcription, DNA-templated chemical synaptic transmission brain development learning memory embryo development regulation of gene expression neuron differentiation neuron projection development multicellular organismal response to stress regulation of gene expression, epigenetic regulation of synaptic plasticity neuromuscular process controlling posture neuromuscular process response to organic cyclic compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MECP2 Polyclonal Antibody

MECP2 Antibody (10861-1-AP) in ICC/IF

Product Details

10861-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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