Invitrogen

MECP2 Polyclonal Antibody

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Antibody Testing Data (9)
Published Figures (5)

MECP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

MECP2 Antibody (PA1-887) in ICC/IF

Immunofluorescence analysis of Mecp2 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MECP2 Polyclonal Antibody (Product # PA1-887) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000), f... View More

MECP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MECP2 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-887

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1500

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

Immunocytochemistry (ICC/IF)

1:100-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fruit fly, Human, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2143845

Product Specific Information

PA1-887 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat tissues and cells.

PA1-887 has been successfully used in Western blot, IHC-P, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.

PA1-887 immunizing peptide corresponds to amino acid residues 1-15 from mouse MeCP2. This sequence is completely conserved in human MeCP2. PA1-887 immunizing peptide (Cat. # PEP-120) is available for use in neutralization and control experiments.

Target Information

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MECP2 impairs neuronal structure by regulating KIBRA.

Neurobiology of disease

Williams AA,White R,Siniard A,Corneveaux J,Huentelman M,Duch C

PA1-887 was used in Western Blotting to identify memory associated KIBRA as a target of MECP2 in regulating dendritic growth.

Fri Jul 01 00:00:00 EDT 2016

Apoptotic Activity of MeCP2 Is Enhanced by C-Terminal Truncating Mutations.

PloS one

Williams AA,Mehler VJ,Mueller C,Vonhoff F,White R,Duch C

PA1-887 was used in western blot study the function of the MeCP2 C-terminus

Mon Jul 31 00:00:00 EDT 2017

Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands.

PloS one

Bueno C,Tabares-Seisdedos R,Moraleda JM,Martinez S

PA1-887 was used in Western Blotting to advance understanding of multiple MeCP2 immunoreactive bands in control neural cells and p.T158M MeCP2e1 mutant cells.

Thu Aug 25 00:00:00 EDT 2016

The Effects of Maternal Separation on Adult Methamphetamine Self-Administration, Extinction, Reinstatement, and MeCP2 Immunoreactivity in the Nucleus Accumbens.

Frontiers in psychiatry

Lewis CR,Staudinger K,Scheck L,Olive MF

PA1-887 was used in immunohistochemistry to study adult methamphetamine self-administration, methamphetamine-seeking behaviour and nucleus accumbens MeCP2 levels following maternal separation in rats

Fri Jun 21 00:00:00 EDT 2013

The first missense mutation causing Rett syndrome specifically affecting the MeCP2_e1 isoform.

Neurogenetics

Fichou Y,Nectoux J,Bahi-Buisson N,Rosas-Vargas H,Girard B,Chelly J,Bienvenu T

PA1-887 was used in immunocytochemistry to investigate a missense mutation in MeCP2_e1 isoform in Rett syndrome

Wed Apr 01 00:00:00 EDT 2009

RNAi-induced down-regulation of Mecp2 expression in the rat brain.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Jin J,Bao X,Wang H,Pan H,Zhang Y,Wu X

PA1-887 was used in western blot to develop a rat model with lowered Mecp2 expression in the brain

Fri Aug 01 00:00:00 EDT 2008

Genetic modifiers of MeCP2 function in Drosophila.

PLoS genetics

Cukier HN,Perez AM,Collins AL,Zhou Z,Zoghbi HY,Botas J

PA1-887 was used in immunohistochemistry and western blot to identify those genes able to compensate for changes in MeCP2's expression level

Fri Sep 05 00:00:00 EDT 2008

A WW domain binding region in methyl-CpG-binding protein MeCP2: impact on Rett syndrome.

Journal of molecular medicine (Berlin, Germany)

Buschdorf JP,Strätling WH

PA1-887 was used in western blot to study the domain structure of methyl-CpG-binding protein MeCP2 and its involvement in Rett syndrome

Sun Feb 01 00:00:00 EST 2004

Multiple pathways regulate MeCP2 expression in normal brain development and exhibit defects in autism-spectrum disorders.

Human molecular genetics

Samaco RC,Nagarajan RP,Braunschweig D,LaSalle JM

PA1-887 was used in immunohistochemistry to study the regulatory mechanisms of the MeCP2 expression during brain development

Mon Mar 15 00:00:00 EST 2004

Quantitative localization of heterogeneous methyl-CpG-binding protein 2 (MeCP2) expression phenotypes in normal and Rett syndrome brain by laser scanning cytometry.

Human molecular genetics

LaSalle JM,Goldstine J,Balmer D,Greco CM

PA1-887 was used in immunohistochemistry to examine the expression profile of methyl-CpG-binding protein 2 in normal and Rett syndrome brains.

Wed Aug 15 00:00:00 EDT 2001

Bioinformatics

Protein Aliases: DKFZp686A24160; MeCp-2 protein; methyl CpG binding protein 2; Methyl-CpG-binding protein 2; testis tissue sperm-binding protein Li 41a

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Gene Aliases: 1500041B07Rik; AUTSX3; D630021H01Rik; Mbd5; MECP2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP10

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UniProt ID: (Human) P51608, (Mouse) Q9Z2D6, (Rat) Q00566

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Entrez Gene ID: (Human) 4204, (Mouse) 17257, (Rat) 29386

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Function(s)

four-way junction DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity mRNA binding protein binding transcription factor binding methyl-CpG binding double-stranded methylated DNA binding protein domain specific binding chromatin DNA binding siRNA binding histone deacetylase binding poly(A) RNA binding unmethylated CpG binding protein N-terminus binding chromatin binding enzyme binding DNA metabolism protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter behavioral fear response response to hypoxia startle response neurological system process involved in regulation of systemic arterial blood pressure regulation of respiratory gaseous exchange by neurological system process inositol metabolic process mitochondrial electron transport, ubiquinol to cytochrome c chromatin silencing regulation of gene expression by genetic imprinting transcription, DNA-templated glutamine metabolic process cellular biogenic amine metabolic process synapse assembly heart development respiratory gaseous exchange long-term memory locomotory behavior protein localization glucocorticoid metabolic process positive regulation of cell proliferation adult locomotory behavior visual learning response to radiation pathogenesis post-embryonic development response to ionizing radiation response to lead ion dendrite development histone methylation histone acetylation proprioception sensory perception of pain cerebellum development ventricular system development negative regulation of histone methylation cardiolipin metabolic process response to estradiol negative regulation of histone acetylation social behavior neuron maturation negative regulation of neuron apoptotic process regulation of DNA methylation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated phosphatidylcholine metabolic process negative regulation of astrocyte differentiation catecholamine secretion positive regulation of synapse assembly excitatory postsynaptic potential long-term synaptic potentiation regulation of transcription, DNA-templated chemical synaptic transmission brain development learning memory embryo development regulation of gene expression neuron differentiation neuron projection development multicellular organismal response to stress regulation of gene expression, epigenetic regulation of synaptic plasticity neuromuscular process controlling posture neuromuscular process response to organic cyclic compound

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MECP2 Polyclonal Antibody

MECP2 Antibody (PA1-887) in ICC/IF

Product Details

PA1-887

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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