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MED27 Antibody (PA5-52374) in WB
Western blot analysis of MED27 in control (vector only transfected HEK293T lysate) and MED27 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MED27 Polyclonal Antibody (Product # PA5-52374).
Product Details
PA5-52374
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human MED27. Recombinant protein control fragment (Product #RP-89375).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2643852
Product Specific Information
Immunogen sequence: KPSENHPLHN SGLLSLDPVQ DKTPLYSQLL QAYKWSNKLQ YHAGLASGLL NQQSLKRSAN QMGVSAKRRP KAQPTTLVLP PQYVDDVISR IDR
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cofactor required for Sp1 transcriptional activation subunit 8; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa; CRSP complex subunit 8; Mediator complex subunit 27; Mediator of RNA polymerase II transcription subunit 27; MGC11274; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34
Gene Aliases: CRAP34; CRSP34; CRSP8; MED27; MED3; TRAP37
UniProt ID: (Human) Q6P2C8
Entrez Gene ID: (Human) 9442
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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