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MERTK Antibody (PA5-95164) in WB
Western blot analysis of MERTK in, Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane a... View More
Product Details
PA5-95164
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MERTK (132-148aa KDGKELLGAHHAITQFY).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2806969
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-mer proto-oncogene tyrosine kinase; MER receptor tyrosine kinase; MGC133349; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase MER; Receptor tyrosine kinase MerTK; Receptor tyrosine tinase gene probably the gene for Rdy; retinal dystrophy; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyro 12; Tyrosine-protein kinase Mer
Gene Aliases: c-Eyk; c-mer; Eyk; MER; MERTK; nmf12; Nyk; rdy; RP38; Tyro12
UniProt ID: (Human) Q12866, (Mouse) Q60805, (Rat) P57097
Entrez Gene ID: (Human) 10461, (Mouse) 17289, (Rat) 65037
natural killer cell differentiation
protein phosphorylation
phagocytosis
cell surface receptor signaling pathway
cell-cell signaling
spermatogenesis
peptidyl-tyrosine phosphorylation
platelet activation
secretion by cell
substrate adhesion-dependent cell spreading
apoptotic cell clearance
protein kinase B signaling
positive regulation of phagocytosis
leukocyte migration
negative regulation of lymphocyte activation
retina development in camera-type eye
vagina development
negative regulation of leukocyte apoptotic process
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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