Invitrogen

MHC Class II (I-A) Monoclonal Antibody (NIMR-4), FITC, eBioscience™

MHC Class II (I-A) Antibody in Flow Cytometry (Flow)

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MHC Class II (I-A) Antibody (11-5322-82) in Flow

Nature communications 2018 - Fig. 9 Physical interaction between TMPs and the DC surface is critical for TMP-mediated DC activation. a Schematic diagram of the transwell system (left). After co-incubation for 2 h, DCs were acid washed and evaluated for TCRbeta and V5G transfer (upper panel), followed continued incubation for 24 h. The expression of MHC class II, CD80, CD86, and CD40 on DCs (CD11c + ) was analysed by flow cytometry an... View More

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Product Details

11-5322-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NIMR-4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465235

Product Specific Information

Description: The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells. MHC Class II molecules are important for the selection of the TCR repertoire during the processes of positive and negative selection. MHC Class II expression is required for the positive selection of CD4+ T cells.

Applications Reported: This NIMR-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NIMR-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 11-5322-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

11-5322 was used in Flow cytometry/Cell sorting to indicate that cell-permeable transgelin-2 have a potential clinical value as a cancer immunotherapy based on DCs.

Wed Mar 17 00:00:00 EDT 2021

Vaccination with early ferroptotic cancer cells induces efficient antitumor immunity.

Journal for immunotherapy of cancer

Efimova I,Catanzaro E,Van der Meeren L,Turubanova VD,Hammad H,Mishchenko TA,Vedunova MV,Fimognari C,Bachert C,Coppieters F,Lefever S,Skirtach AG,Krysko O,Krysko DV

11-5322-82 was used in Flow Cytometry to pave the way for the development of new therapeutic strategies for cancers based on induction of ferroptosis, and thus broadens the current concept of immunogenic cell death and opens the door for the development of new strategies in cancer immunotherapy.

Sun Nov 01 00:00:00 EDT 2020

Immunoadjuvant Activity of Fucoidans from the Brown Alga Fucus evanescens.

Marine drugs

Kuznetsova TA,Smolina TP,Makarenkova ID,Ivanushko LA,Persiyanova EV,Ermakova SP,Silchenko AS,Zaporozhets TS,Besednova NN,Fedyanina LN,Kryzhanovsky SP

11-5322 was used in Flow cytometry/Cell sorting to comparatively evaluate the effect of fucoidans from the brown alga Fucus evanescens on effector functions of innate and adaptive immunity cells in vitro and in vivo.

Wed Mar 11 00:00:00 EDT 2020

PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani.

Infection and immunity

Habib S,El Andaloussi A,Elmasry K,Handoussa A,Azab M,Elsawey A,Al-Hendy A,Ismail N

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 11-5322-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.

Nature communications

Kim HR,Mun Y,Lee KS,Park YJ,Park JS,Park JH,Jeon BN,Kim CH,Jun Y,Hyun YM,Kim M,Lee SM,Park CS,Im SH,Jun CD

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 11-5322-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

Transcriptional determinants of tolerogenic and immunogenic states during dendritic cell maturation.

The Journal of cell biology

Vander Lugt B,Riddell J,Khan AA,Hackney JA,Lesch J,DeVoss J,Weirauch MT,Singh H,Mellman I

11532282 was used in flow cytometry to elucidate the role of interferon regulatory factor 4 in dendritic cells

Mon Mar 06 00:00:00 EST 2017

Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model.

The Prostate

Sulek JE,Robinson SP,Petrossian AA,Zhou S,Goliadze E,Manjili MH,Toor A,Guruli G

11532282 was used in flow cytometry to study cancer-testis antigen expression in a mouse model of prostate cancer

Wed Mar 01 00:00:00 EST 2017

Mouse IP-10 Gene Delivered by Folate-modified Chitosan Nanoparticles and Dendritic/tumor Cells Fusion Vaccine Effectively Inhibit the Growth of Hepatocellular Carcinoma in Mice.

Theranostics

Hu Z,Chen J,Zhou S,Yang N,Duan S,Zhang Z,Su J,He J,Zhang Z,Lu X,Zhao Y

11-5322 was used in Flow cytometry/Cell sorting to illustrate that the mIP-10 enhances the anti-tumor effect of DC/tumor cell fusion vaccine by alleviating the immunosuppressive tumor environment.

Mon Mar 19 00:00:00 EDT 2018

Lymphatic vessels regulate immune microenvironments in human and murine melanoma.

The Journal of clinical investigation

Lund AW,Wagner M,Fankhauser M,Steinskog ES,Broggi MA,Spranger S,Gajewski TF,Alitalo K,Eikesdal HP,Wiig H,Swartz MA

11-5322 was used in Immunohistochemistry to suggest that the establishment of tumour-associated inflammation and immunity critically depends on lymphatic vessel remodelling and drainage.

Thu Sep 01 00:00:00 EDT 2016

Pro-inflammatory effect of a traditional Chinese medicine formula with potent anti-cancer activity in vitro impedes tumor inhibitory potential in vivo.

Molecular and clinical oncology

Xia L,Plachynta M,Liu T,Xiao X,Song J,Li X,Zhang M,Yao Y,Luo H,Hao X,Ben-David Y

11-5322 was used in Flow cytometry/Cell sorting to highlight the complexity of Chinese medicinal compounds and the need to thoroughly analyze their systemic effects at high concentrations in vivo.

Thu Dec 01 00:00:00 EST 2016

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 11-5322-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Lung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor.

Experimental and therapeutic medicine

Qin Q,Wang Z,Pan P,Cao Z,Xia Q,Tan H,Hu C

Published figure using MHC Class II (I-A) monoclonal antibody (Product # 11-5322-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

Nocardia brasiliensis cell wall lipids modulate macrophage and dendritic responses that favor development of experimental actinomycetoma in BALB/c mice.

Infection and immunity

Trevino-Villarreal JH,Vera-Cabrera L,Valero-Guillén PL,Salinas-Carmona MC

11-5322 was used in Flow cytometry/Cell sorting to investigate the parthenogenesis of Nocardia brasiliensis infection, showing that N.brasiliensis cell wall lipids modulate macrophage and dendritic responses, leading to actinomycetoma.

Mon Oct 01 00:00:00 EDT 2012

Th-1 lymphocytes induce dendritic cell tumor killing activity by an IFN-γ-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

LaCasse CJ,Janikashvili N,Larmonier CB,Alizadeh D,Hanke N,Kartchner J,Situ E,Centuori S,Har-Noy M,Bonnotte B,Katsanis E,Larmonier N

11-5322 was used in Flow cytometry/Cell sorting to investigate the regulation of murine dendritic cell cytotoxic functions by T lymphocytes.

Thu Dec 15 00:00:00 EST 2011

Rapid immunomagnetic negative enrichment of neutrophil granulocytes from murine bone marrow for functional studies in vitro and in vivo.

PloS one

Hasenberg M,Köhler A,Bonifatius S,Borucki K,Riek-Burchardt M,Achilles J,Männ L,Baumgart K,Schraven B,Gunzer M

11-5322 was used in Flow cytometry/Cell sorting to develop a novel protocol for isolating pure polymorphonuclear neutrophils from murine bone marrow.

Wed Feb 23 00:00:00 EST 2011

A subpopulation of mesenchymal stromal cells with high osteogenic potential.

Journal of cellular and molecular medicine

Liu H,Toh WS,Lu K,MacAry PA,Kemeny DM,Cao T

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: H-2 class II histocompatibility antigen, A beta chain; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

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Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

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UniProt ID: (Mouse) P14483

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Entrez Gene ID: (Mouse) 14961

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Function(s)

protein binding transcription factor binding toxic substance binding ubiquitin protein ligase binding peptide antigen binding protein heterodimerization activity repressing transcription factor binding major histocompatibility complex protein

Process(es)

B cell affinity maturation immune system process immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin antigen processing and presentation of peptide or polysaccharide antigen via MHC class II positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of T cell proliferation positive regulation of alpha-beta T cell activation antigen processing and presentation of peptide antigen cellular response to interferon-gamma

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class II (I-A) Monoclonal Antibody (NIMR-4), FITC, eBioscience™

MHC Class II (I-A) Antibody (11-5322-82) in Flow

Product Details

11-5322-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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