Invitrogen

MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), eFluor™ 450, eBioscience™

6 Published Figures

16 References

View all (23) MHC Class II (I-Ab) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

MHC Class II I-Ab Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

MHC Class II I-Ab Antibody (48-5320-82) in Flow

Staining of C57Bl/6 splenocytes with 0.5 µg of Mouse IgG2a K Isotype Control eFluor® 450 (Product # 48-4724-82) (blue histogram) or 0.5 µg of Anti-Mouse MHC Class II I-Ab eFluor® 450 (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

48-5320-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AF6-120.1

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10669941

Product Specific Information

Description: This AF6-120.1 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4.

The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed.

Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

I-Ab (H2-Ab1, histocompatibility 2, class II antigen A, beta 1) is an MHC class II heterodimer molecule of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. H2-Ab1 heterodimer chains alpha (DQA) and beta (DQB) are both anchored in the membrane. H2-Ab1 plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for polymorphisms is routinely done for bone marrow transplantation.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

DNASE1L3 enhances antitumor immunity and suppresses tumor progression in colon cancer.

JCI insight

Li W,Nakano H,Fan W,Li Y,Sil P,Nakano K,Zhao F,Karmaus PW,Grimm SA,Shi M,Xu X,Mizuta R,Kitamura D,Wan Y,Fessler MB,Cook DN,Shats I,Li X,Li L

48-5320-82 was used in Flow cytometry/Cell sorting to identify DNASE1L3 as a potentially new regulator of antitumor immunity and a tumor suppressor in colon cancer.

Fri Sep 08 00:00:00 EDT 2023

Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin.

Cell death and differentiation

Velletri T,Huang Y,Wang Y,Li Q,Hu M,Xie N,Yang Q,Chen X,Chen Q,Shou P,Gan Y,Candi E,Annicchiarico-Petruzzelli M,Agostini M,Yang H,Melino G,Shi Y,Wang Y

Published figure using MHC Class II I-Ab monoclonal antibody (Product # 48-5320-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Gut microbial dysbiosis after traumatic brain injury modulates the immune response and impairs neurogenesis.

Acta neuropathologica communications

Celorrio M,Abellanas MA,Rhodes J,Goodwin V,Moritz J,Vadivelu S,Wang L,Rodgers R,Xiao S,Anabayan I,Payne C,Perry AM,Baldridge MT,Aymerich MS,Steed A,Friess SH

Published figure using MHC Class II I-Ab monoclonal antibody (Product # 48-5320-82) in Flow Cytometry

Wed Mar 10 00:00:00 EST 2021

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

Published figure using MHC Class II I-Ab monoclonal antibody (Product # 48-5320-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis.

Cell

Kitamoto S,Nagao-Kitamoto H,Jiao Y,Gillilland MG,Hayashi A,Imai J,Sugihara K,Miyoshi M,Brazil JC,Kuffa P,Hill BD,Rizvi SM,Wen F,Bishu S,Inohara N,Eaton KA,Nusrat A,Lei YL,Giannobile WV,Kamada N

48-5320-82 was used in Flow Cytometry to conclude that oral inflammation, such as periodontitis, exacerbates gut inflammation by supplying the gut with both colitogenic pathobionts and pathogenic T cells.

Thu Jul 23 00:00:00 EDT 2020

Resident macrophages acquire innate immune memory in staphylococcal skin infection.

eLife

Feuerstein R,Forde AJ,Lohrmann F,Kolter J,Ramirez NJ,Zimmermann J,Gomez de Agüero M,Henneke P

48-5320 was used in Flow cytometry/Cell sorting to show that resident dermal Mφ are programmed locally, independently of bone marrow-derived monocytes during staphylococcal skin infection leading to transiently increased resistance against a second infection.

Wed Jul 08 00:00:00 EDT 2020

Small Molecule Enhancers of Endosome-to-Cytosol Import Augment Anti-tumor Immunity.

Cell reports

Kozik P,Gros M,Itzhak DN,Joannas L,Heurtebise-Chrétien S,Krawczyk PA,Rodríguez-Silvestre P,Alloatti A,Magalhaes JG,Del Nery E,Borner GHH,Amigorena S

48-5320 was used in Flow cytometry/Cell sorting to identify 37 enhancers of antigen import from endolysosomes into the cytosol from screening 700 US Food and Drug Administration (FDA)-approved drugs and reveal their mechanism of action.

Tue Jul 14 00:00:00 EDT 2020

Chaperone protein HSC70 regulates intercellular transfer of Y chromosome antigen DBY.

The Journal of clinical investigation

Kretschmann S,Herda S,Bruns H,Russ J,van der Meijden ED,Schlötzer-Schrehardt U,Griffioen M,Na IK,Mackensen A,Kremer AN

48-5320-82 was used in Flow Cytometry to show that intercellular antigen transfer of human Y chromosome antigen DBY is tightly regulated via binding to HSC70, and that this mechanism influences recognition and rejection of the MHC-II negative tumours in vivo.

Mon Jun 17 00:00:00 EDT 2019

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using MHC Class II I-Ab monoclonal antibody (Product # 48-5320-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Critical role for Sec22b-dependent antigen cross-presentation in antitumor immunity.

The Journal of experimental medicine

Alloatti A,Rookhuizen DC,Joannas L,Carpier JM,Iborra S,Magalhaes JG,Yatim N,Kozik P,Sancho D,Albert ML,Amigorena S

48-5320 was used in Flow cytometry/Cell sorting to determine that Sec22b-dependent cross-presentation in dendritic cells is required to initiate CD8 T cell responses to dead cells, and induce effective anti-tumour immune responses during anti-PD-1 treatment.

Mon Aug 07 00:00:00 EDT 2017

FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.

Frontiers in immunology

Pan YG,Yu YL,Lin CC,Lanier LL,Chu CL

Published figure using MHC Class II I-Ab monoclonal antibody (Product # 48-5320-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

48-5320 was used in Flow cytometry/Cell sorting to study how immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Mon Dec 01 00:00:00 EST 2014

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

48-5320 was used in Flow cytometry/Cell sorting to study how immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Mon Dec 01 00:00:00 EST 2014

Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.

PloS one

Danzl NM,Jeong S,Choi Y,Alexandropoulos K

48-5320 was used in Immunocytochemistry to examine the developmental stages of thymic epithelial cells, identifying novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.

Wed Oct 29 00:00:00 EDT 2014

Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.

PloS one

Danzl NM,Jeong S,Choi Y,Alexandropoulos K

Wed Oct 29 00:00:00 EDT 2014

Reduced production of B-1-specified common lymphoid progenitors results in diminished potential of adult marrow to generate B-1 cells.

Proceedings of the National Academy of Sciences of the United States of America

Barber CL,Montecino-Rodriguez E,Dorshkind K

Tue Aug 16 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CELIAC1; DADB-249P12.2; H-2 class II histocompatibility antigen, A beta chain; H-2Eb; H2Eb; histocompatibility 2, class II antigen A, beta 1; HLA-DQB; Ia-4; Ia4; IDDM1; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

View more View less

Gene Aliases: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

View more View less

UniProt ID: (Mouse) P14483

View more View less

Entrez Gene ID: (Mouse) 14961

View more View less

Function(s)

protein binding transcription factor binding toxic substance binding ubiquitin protein ligase binding peptide antigen binding protein heterodimerization activity repressing transcription factor binding major histocompatibility complex protein

Process(es)

B cell affinity maturation immune system process immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin antigen processing and presentation of peptide or polysaccharide antigen via MHC class II positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II negative regulation of T cell proliferation positive regulation of alpha-beta T cell activation antigen processing and presentation of peptide antigen cellular response to interferon-gamma

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), eFluor™ 450, eBioscience™

MHC Class II I-Ab Antibody (48-5320-82) in Flow

Product Details

48-5320-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!