Invitrogen

CD105 Monoclonal Antibody (SN6), Alexa Fluor™ 488

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

7 References

View all (127) CD105 (Endoglin) antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 7

CD105 Antibody (MHCD10520) in Flow

U937 cells were stained with Alexa Fluor® 488-conjugated anti-human CD105 monoclonal antibody (Product # MHCD10520). The negative profile represents unstained cells.

Product Image Gallery

CD105 Antibody — Fig. 4 Involvement of alphaIIbbeta3 integrin in endoglin-mediated cell adhesion. a Adhesion of platelets and CHO-alphaIIbbeta3 cells to endoglin-expressing cells was measured under flow. Calcein-labeled platelets or CHO-alphaIIbbeta3 cells were perfused in flow chambers previously coated with parental (L6E9-P), mock-transfected (L6E9-M), L-endoglin-transfected (L6E9-L) or S-endoglin-transfected (L6E9-S) cells in the absence or presence of CXCL12, allowed to adhere in the absence of flow for 10 min, and then subjected to 2 dynes/cm 2 for 2 min. Quantification of adhesion, repeated in triplicates with different platelets (dark green) or CHO cells (light green) preparations, was performed by ImageJ software. Mean values +- SEM are displayed. ** p < 0.005; NS not significant. Parallel experiments using calcein-labeled CHO wild-type cells did not show any significant adhesion to endoglin-expressing L6E9 transfectants (fluorescence levels < 2; data not shown). b Western blot analysis of endoglin in L6E9 mock, L-endoglin (L-Eng) and S-endoglin (S-Eng) transfectants, using tubulin as a loading control. c Representative experiment of adhesion of calcein-labeled CHO-alphaIIbbeta3 cells to flow chambers coated with mock-transfected cells (L6E9-M) or L-endoglin-transfected cells (L6E9-L), performed as described in a and visualized by fluorescence microscopy (x10 magnification). Scale bars, 20 um. d , e Platelet adhesion to endoglin is inhibited by anti-beta3 antibodies. Plates coated wit {TM}

Product Details

MHCD10520

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SN6

Immunogen

Human CD105 (Endoglin)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373111

Product Specific Information

Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The effects of 3D culture on the expansion and maintenance of nucleus pulposus progenitor cell multipotency.

JOR spine

Guerrero J,Häckel S,Croft AS,Albers CE,Gantenbein B

Published figure using CD105 monoclonal antibody (Product # MHCD10520) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.

PloS one

Burns AB,Doris C,Vehar K,Saxena V,Bardliving C,Shamlou PA,Phillips MI

Published figure using CD105 monoclonal antibody (Product # MHCD10520) in Flow Cytometry

Wed Nov 17 00:00:00 EST 2021

Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.

PloS one

Burns AB,Doris C,Vehar K,Saxena V,Bardliving C,Shamlou PA,Phillips MI

Published figure using CD105 monoclonal antibody (Product # MHCD10520) in Flow Cytometry

Wed Nov 17 00:00:00 EST 2021

Human endoglin as a potential new partner involved in platelet-endothelium interactions.

Cellular and molecular life sciences : CMLS

Rossi E,Pericacho M,Bachelot-Loza C,Pidard D,Gaussem P,Poirault-Chassac S,Blanco FJ,Langa C,González-Manchón C,Novoa JML,Smadja DM,Bernabeu C

Published figure using CD105 monoclonal antibody (Product # MHCD10520) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Myogenic potential of human alveolar mucosa derived cells.

Cell cycle (Georgetown, Tex.)

Zorin VL,Pulin AA,Eremin II,Korsakov IN,Zorina AI,Khromova NV,Sokova OI,Kotenko KV,Kopnin PB

MHCD10520 was used in flow cytometry to evaluate the myogenic potential and proliferative capacity of human alveolar mucosa cells

Sun Mar 19 00:00:00 EDT 2017

Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.

Journal of tissue engineering and regenerative medicine

Zorin V,Zorina A,Cherkasov V,Deev R,Kopnin P,Isaev A

MHCD10520 was used in flow cytometry to study the safety and clinical effectiveness of dermal autofibroblast therapy.

Wed Mar 01 00:00:00 EST 2017

Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.

Biomedical materials (Bristol, England)

Zorin VL,Komlev VS,Zorina AI,Khromova NV,Solovieva EV,Fedotov AY,Eremin II,Kopnin PB

MHCD10520 was used in flow cytometry to evaluate the osteogenic capacity of bone marrow mesenchymal stream cells and gingiva-derived mesenchymal stromal cells

Thu Aug 28 00:00:00 EDT 2014

Time-lapse microscopy and classification of 2D human mesenchymal stem cells based on cell shape picks up myogenic from osteogenic and adipogenic differentiation.

Journal of tissue engineering and regenerative medicine

Seiler C,Gazdhar A,Reyes M,Benneker LM,Geiser T,Siebenrock KA,Gantenbein-Ritter B

MHCD10520 was used in flow cytometry to suggest that non-invasive automated time-lapse microscopy could potentially be used to predict the stem cell fate of human mesenchymal stem cells (hMSCs) for clinical application, based on morphology for earlier time-points.

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD105; CD105 antigen; Endoglin; ORW

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Gene Aliases: END; ENG; HHT1; ORW1

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UniProt ID: (Human) P17813

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Entrez Gene ID: (Human) 2022

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Function(s)

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding transmembrane signal receptor

Process(es)

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD105 Monoclonal Antibody (SN6), Alexa Fluor™ 488

CD105 Antibody (MHCD10520) in Flow

Product Details

MHCD10520

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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