Invitrogen

CD19 Monoclonal Antibody (SJ25-C1), PE

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

8 References

View all (322) CD19 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Advanced Verification (1)

FIGURE: 1 / 2

CD19 Antibody (MHCD1904) in Flow

Anti-human CD19 monoclonal antibody (clone SJ25-C1) was used to delineate B-cells in a human peripheral blood lymphocyte gate. R-PE is shown, and corresponding negative control profiles represent unstained cells.

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD1904

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SJ25-C1

Immunogen

Nalm 1 Human cell line

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373382

Product Specific Information

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies.

Journal of visualized experiments : JoVE

Xia X,Li H,Satheesan S,Zhou J,Rossi JJ

MHCD1904 was used in Flow Cytometry to report a detailed protocol for generating a humanized NSG mouse model (hu-NSG) by hematopoietic stem cell transplantation into a radiation-conditioned neonatal NSG mouse.

Mon Jan 07 00:00:00 EST 2019

Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma.

The Journal of pathology

Strickland LA,Ross J,Williams S,Ross S,Romero M,Spencer S,Erickson R,Sutcliffe J,Verbeke C,Polakis P,van Bruggen N,Koeppen H

MHCD1904 was used in flow cytometry to investigate targeting CEACAM6 for treatment of pancreatic adenocarcinomas.

Wed Jul 01 00:00:00 EDT 2009

Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice.

Haematologica

Timeus F,Crescenzio N,Sanavio F,Fazio L,Doria A,Foglia L,Pignochino Y,Berger M,Piacibello W,Madon E,Cordero di Montezemolo L,Fagioli F

MHCD1904 was used in flow cytometry assess the capacity of cord blood stem cells to repopulate irradiated NOD/SCID mice after cryopreservation

Wed Mar 01 00:00:00 EST 2006

Omalizumab treatment downregulates dendritic cell FcepsilonRI expression.

The Journal of allergy and clinical immunology

Prussin C,Griffith DT,Boesel KM,Lin H,Foster B,Casale TB

MHCD1904 was used in Flow cytometry/Cell sorting to analyse cell surface expression of FcepsilonRIalpha on precursor dendritic cells from seasonal allergic rhinitis patients treated with omalizumab.

Mon Dec 01 00:00:00 EST 2003

Adiponectin, a fat cell product, influences the earliest lymphocyte precursors in bone marrow cultures by activation of the cyclooxygenase-prostaglandin pathway in stromal cells.

Journal of immunology (Baltimore, Md. : 1950)

Yokota T,Meka CS,Kouro T,Medina KL,Igarashi H,Takahashi M,Oritani K,Funahashi T,Tomiyama Y,Matsuzawa Y,Kincade PW

MHCD1904 was used in flow cytometry to test if adiponectin affects lymphohematopoiesis.

Sat Nov 15 00:00:00 EST 2003

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Cytometry. Part B, Clinical cytometry

Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G

MHCD1904 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.

Tue Jul 01 00:00:00 EDT 2003

Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors.

Blood

McIlroy D,Troadec C,Grassi F,Samri A,Barrou B,Autran B,Debré P,Feuillard J,Hosmalin A

MHCD1904 was used in flow cytometry to characterize human splenic dendritic cells.

Fri Jun 01 00:00:00 EDT 2001

Enhanced myeloid specificity of CD117 compared with CD13 and CD33.

Leukemia research

Nomdedéu JF,Mateu R,Altès A,Llorente A,Rio C,Estivill C,López O,Ubeda J,Rubiol E

MHCD1904 was used in flow cytometry to compare the expression pattern of CD13, CD33, and CD117 in patients with acute myelogenous leukemia.

Thu Apr 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

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Gene Aliases: B4; CD19; CVID3

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UniProt ID: (Human) P15391

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Entrez Gene ID: (Human) 930

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Function(s)

receptor signaling protein activity protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

cellular defense response cell surface receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of immune response B cell receptor signaling pathway

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CD19 Monoclonal Antibody (SJ25-C1), PE

CD19 Antibody (MHCD1904) in Flow

Product Details

MHCD1904

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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