Invitrogen

CD24 Monoclonal Antibody (SN3), FITC

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 References

View all (96) CD24 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Advanced Verification (1)

FIGURE: 1 / 1

CD24 Antibody (MHCD2401)

Figure 3. CT targets LNCaP TICs. (A) FACS analysis was performed to determine the percentage of CD44+CD24- cells after treatment with increasing doses of CT (2.5, 5 and 10 uM). Data from a representative experiment (from a total of three) are shown. (B) Percentage of CD44 + CD24 - LNCaP cells compared to untreated control. The percentage reduced in a dose dependent manner, however no statistically significa... View More

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CD24 Antibody — Figure 3. CT targets LNCaP TICs. (A) FACS analysis was performed to determine the percentage of CD44+CD24- cells after treatment with increasing doses of CT (2.5, 5 and 10 uM). Data from a representative experiment (from a total of three) are shown. (B) Percentage of CD44 + CD24 - LNCaP cells compared to untreated control. The percentage reduced in a dose dependent manner, however no statistically significant difference was observed between the control and CT treated groups. (C) Sphere formation assay quantification was performed using increasing doses of CT. (D) The sphere forming ability was significantly inhibited by CT while the dose up to 1.5 uM, (*P<0.05; **P<0.01). (E) Sphere formation assay of CD44 + /CD24 - cells was performed using increasing doses of CT. (F) The sphere forming ability were inhibited by CT while the dose up to 0.625 uM (**P<0.05; *P<0.01). FACS, fluorescent activated cell sorting; CT, cryptotanshinone; TICs, tumor initiating cells. {TM}

Product Details

MHCD2401

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SN3

Immunogen

Human CD24

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372045

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

Analyte Specific Reagent

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Product References

Three-Dimensional Cellular Arrangement in Epithelial Ovarian Cancer Cell Lines TOV-21G and SKOV-3 is Associated with Apoptosis-Related miRNA Expression Modulation.

Cancer microenvironment : official journal of the International Cancer Microenvironment Society

de Lima AB,Silva LM,Gonçales NG,Carvalho MRS,da Silva Filho AL,da Conceição Braga L

MHCD2401 was used in Flow cytometry/Cell sorting to evaluate the tumoursphere model as a cancer stem cell-enrichment method in epithelial ovarian cancer studies, and investigate apoptosis-related miRNAs in tumourspheres-derived epithelial ovarian cancer cell lines.

Fri Jun 01 00:00:00 EDT 2018

New 3-alkylpyridine marine alkaloid analogues as promising antitumor agents against the CD44^+/high /CD24^-/low subset of triple-negative breast cancer cell line.

Chemical biology & drug design

de Lima AB,Barbosa CS,Gonçalves AMMN,Santos FVD,Viana GHR,Varotti FP,Silva LM

MHCD2401 was used in flow cytometry to suggest that synthetic analogues of Theonelladin C are a promising class for development of anticancer agents

Sat Jul 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); FLJ22950; FLJ43543; MGC75043; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

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Gene Aliases: CD24; CD24A

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UniProt ID: (Human) P25063

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Entrez Gene ID: (Human) 100133941

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Function(s)

signal transducer activity protein binding protein kinase binding protein tyrosine kinase activator activity

Process(es)

response to hypoxia cell activation regulation of cytokine-mediated signaling pathway response to molecule of bacterial origin immune response-regulating cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration Wnt signaling pathway single organismal cell-cell adhesion cell migration regulation of epithelial cell differentiation T cell costimulation B cell receptor transport into membrane raft chemokine receptor transport out of membrane raft negative regulation of transforming growth factor beta3 production positive regulation of activated T cell proliferation regulation of phosphorylation cholesterol homeostasis positive regulation of MAP kinase activity regulation of MAPK cascade response to estrogen respiratory burst positive regulation of protein tyrosine kinase activity glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation intrinsic apoptotic signaling pathway positive regulation of nephron tubule epithelial cell differentiation

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CD24 Monoclonal Antibody (SN3), FITC

CD24 Antibody (MHCD2401)

Product Details

MHCD2401

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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