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Invitrogen
CD45RO Monoclonal Antibody (UCHL1), APC
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 1
CD45RO Antibody (MHCD45RO05)
Antibody clone (UCHL1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in UCHL1 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45RO antibody (UCHL1). Yellow histogram represents staining with the isotype control. {KO}
Product Details
MHCD45RO05
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
UCHL1
Immunogen
Human CD45RO
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS with BSA, sucrose
Contains
0.1% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10372517
Product Specific Information
Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.
Target Information
CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Analyte Specific Reagent
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Bioinformatics
Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200
UniProt ID: (Human) P08575
Entrez Gene ID: (Human) 5788
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
immunoglobulin biosynthetic process
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
cell surface receptor signaling pathway
dephosphorylation
T cell differentiation
positive regulation of B cell proliferation
peptidyl-tyrosine dephosphorylation
B cell proliferation
positive regulation of T cell proliferation
cell cycle phase transition
positive regulation of protein kinase activity
bone marrow development
stem cell development
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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