Invitrogen

CXCR4 Monoclonal Antibody (12G5), PE

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5 Published Figures

16 References

View all (76) CXCR4 (CD184) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (5)
Advanced Verification (1)

FIGURE: 1 / 6

CXCR4 Antibody (MHCXCR404) in Flow

The Journal of pathology 2023 - 2 Figure KRT19 is dispensable for PP and PDLO formation. (A) Scheme of KRT19 knockout (KO) cell line generation and differentiation to PPs and PDLOs. (B) Flow cytometry of respective differentiation markers of WT and KRT19 (KO) cell lines at DE (stage markers CXCR4 and KIT), PE (stage marker PDX1), and PP (markers NKX6-1 and PDX1) stage of differentiation. N = 3 independent differentiations with two cell li... View More

Product Image Gallery

CXCR4 Antibody — CXCR4 expression levels on CEM.NKR cells after various treatments. CEM.NKR cells were left untreated, treated with SWNTs only or SWNTS conjugated to a luciferase (Luc) siRNA control (SWNT-siRNAluc). Cells were also treated with SWNTs conjugated to two different CXCR4 siRNAs: SWNT-siRNACXCR4a or SWNT-siRNACXCR4b. Both siRNACXCR4a and siRNACXCR4b target CXCR4 mRNA but have different sequences. Cells were also treated with SWNT-siRNACXCR4a in combination with four types of commercial cationic liposome agents (Lipo1-4). The siRNA concentration used was 500 nM in the incubation solution, SWNTs were used at a concentration of 10 mg l-1. Cells were incubated for 3 d before the analysis of CXCR4 expression levels using FACS analysis. Human T cells are not transfected by liposome-based transfection agents. In contrast, the SWNT-conjugated siRNA transfection method was highly efficient at targeting CXCR4. The error bars represent the standard deviation (s.d.) of three samples. This figure is adapted from our previously published work and reproduced with permission from Wiley InterScience4. {KD}

Product Details

MHCXCR404

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

12G5

Immunogen

SIV-infected SupT1 cells injected into Balb/c mice

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373097

Product Specific Information

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

Analyte Specific Reagent

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Product References

DNA methylation-associated allelic inactivation regulates Keratin 19 gene expression during pancreatic development and carcinogenesis.

The Journal of pathology

Krüger J,Fischer A,Breunig M,Allgöwer C,Schulte L,Merkle J,Mulaw MA,Okeke N,Melzer MK,Morgenstern C,Azoitei N,Seufferlein T,Barth TF,Siebert R,Hohwieler M,Kleger A

Published figure using CXCR4 monoclonal antibody (Product # MHCXCR404) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2023

Functional Genomic Screening in Human Pluripotent Stem Cells Reveals New Roadblocks in Early Pancreatic Endoderm Formation.

Cells

Krüger J,Breunig M,Pasquini LP,Morawe M,Groß A,Arnold F,Russell R,Seufferlein T,Azoitei N,Kestler HA,Julier C,Heller S,Hohwieler M,Kleger A

Published figure using CXCR4 monoclonal antibody (Product # MHCXCR404) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells.

Science advances

Werder RB,Liu T,Abo KM,Lindstrom-Vautrin J,Villacorta-Martin C,Huang J,Hinds A,Boyer N,Bullitt E,Liesa M,Silverman EK,Kotton DN,Cho MH,Zhou X,Wilson AA

MHCXCR404 was used in Flow cytometry/Cell sorting to apply CRISPR interference to interrogate the function of nine genes implicated in COPD by GWAS in induced pluripotent stem cell-derived type 2 alveolar epithelial cells.

Fri Jul 15 00:00:00 EDT 2022

Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells.

Cell stem cell

Breunig M,Merkle J,Wagner M,Melzer MK,Barth TFE,Engleitner T,Krumm J,Wiedenmann S,Cohrs CM,Perkhofer L,Jain G,Krüger J,Hermann PC,Schmid M,Madácsy T,Varga Á,Griger J,Azoitei N,Müller M,Wessely O,Robey PG,Heller S,Dantes Z,Reichert M,Günes C,Bolenz C,Kuhn F,Maléth J,Speier S,Liebau S,Sipos B,Kuster B,Seufferlein T,Rad R,Meier M,Hohwieler M,Kleger A

MHCXCR404 was used in Flow cytometry/Cell sorting to show that PDLOs enable in vitro and in vivo studies of pancreatic plasticity, dysplasia, and cancer formation from a genetically defined background.

Thu Jun 03 00:00:00 EDT 2021

Differentiation of human pluripotent stem cells into pancreatic duct-like organoids.

STAR protocols

Breunig M,Merkle J,Melzer MK,Heller S,Seufferlein T,Meier M,Hohwieler M,Kleger A

Published figure using CXCR4 monoclonal antibody (Product # MHCXCR404) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Adenine base editing reduces misfolded protein accumulation and toxicity in alpha-1 antitrypsin deficient patient iPSC-hepatocytes.

Molecular therapy : the journal of the American Society of Gene Therapy

Werder RB,Kaserman JE,Packer MS,Lindstrom-Vautrin J,Villacorta-Martin C,Young LE,Aratyn-Schaus Y,Gregoire F,Wilson AA

MHCXCR404 was used in Flow cytometry/Cell sorting to apply adenine base editors to correct the Z mutation, a single-base substitution that leads to AAT protein misfolding and associated liver and lung disease, in patient induced pluripotent stem cells (iPSCs) and iPSC-derived hepatocytes (iHeps).

Wed Nov 03 00:00:00 EDT 2021

Transcriptional changes and the role of ONECUT1 in hPSC pancreatic differentiation.

Communications biology

Heller S,Li Z,Lin Q,Geusz R,Breunig M,Hohwieler M,Zhang X,Nair GG,Seufferlein T,Hebrok M,Sander M,Julier C,Kleger A,Costa IG

MHCXCR404 was used in Flow Cytometry to suggest that ONECUT1 is also involved in preparing pancreatic progenitors for later endocrine specification.

Wed Nov 17 00:00:00 EST 2021

CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome.

Cancers

Merkle J,Breunig M,Schmid M,Allgöwer C,Krüger J,Melzer MK,Bens S,Siebert R,Perkhofer L,Azoitei N,Seufferlein T,Heller S,Meier M,Müller M,Kleger A,Hohwieler M

MHCXCR404 was used in Flow Cytometry to provide a model for hereditary human pancreatic cancer that enables dissection of tumor initiation and early development starting from patient-specific CDKN2A-mutated pluripotent stem cells.

Wed Oct 13 00:00:00 EDT 2021

Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells.

Nature communications

Mithal A,Capilla A,Heinze D,Berical A,Villacorta-Martin C,Vedaie M,Jacob A,Abo K,Szymaniak A,Peasley M,Stuffer A,Mahoney J,Kotton DN,Hawkins F,Mostoslavsky G

MHCXCR404 was used in Flow cytometry/Cell sorting to report a directed differentiation protocol for the generation of mesenchyme-free HIOs that can be primed towards more colonic or proximal intestinal lineages in serum-free defined conditions.

Fri Jan 10 00:00:00 EST 2020

Clampdown of inflammation in aging and anticancer therapies by limiting upregulation and activation of GPCR, CXCR4.

NPJ aging and mechanisms of disease

Nair RR,Madiwale SV,Saini DK

MHCXCR404 was used in Immunocytochemistry-immunoflourescence to identify a unique DNA damage-linked inflammatory cascade, mediated by expression level changes in a GPCR and which can be targeted to counteract inflammation during anti-cancer therapies and ageing.

Thu Oct 01 00:00:00 EDT 2020

Clampdown of inflammation in aging and anticancer therapies by limiting upregulation and activation of GPCR, CXCR4.

NPJ aging and mechanisms of disease

Nair RR,Madiwale SV,Saini DK

Thu Oct 01 00:00:00 EDT 2020

Genome Editing of the CYP1A1 Locus in iPSCs as a Platform to Map AHR Expression throughout Human Development.

Stem cells international

Smith BW,Stanford EA,Sherr DH,Murphy GJ

Published figure using CXCR4 monoclonal antibody (Product # MHCXCR404) in Flow Cytometry

Thu May 05 00:00:00 EDT 2016

Multisystemic Disease Modeling of Liver-Derived Protein Folding Disorders Using Induced Pluripotent Stem Cells (iPSCs).

Methods in molecular biology (Clifton, N.J.)

Leung A,Murphy GJ

MHCXCR404 was used in immunohistochemistry to describe protocols to directed differentiation of protein folding disease-specific iPSCs into hepatocytes and neural-lineage cells.

Thu Aug 18 00:00:00 EDT 2016

The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.

Nature protocols

Huang SX,Green MD,de Carvalho AT,Mumau M,Chen YW,D'Souza SL,Snoeck HW

MHCXCR404 was used in Immunofluorescence to describe a strategy for directed differentiation of hPSCs into developmental lung progenitors, and their subsequent differentiation into predominantly distal lung epithelial cells.

Sun Mar 01 00:00:00 EST 2015

KPNA2 expression is an independent adverse predictor of biochemical recurrence after radical prostatectomy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Mortezavi A,Hermanns T,Seifert HH,Baumgartner MK,Provenzano M,Sulser T,Burger M,Montani M,Ikenberg K,Hofstädter F,Hartmann A,Jaggi R,Moch H,Kristiansen G,Wild PJ

MHCXCR404 was used in immunohistochemistry - paraffin section to examine karyopherin alpha 2 expression in prostate cancer samples.

Tue Mar 01 00:00:00 EST 2011

Co-culture of hematopoietic stem cells with mesenchymal stem cells increases VCAM-1-dependent migration of primitive hematopoietic stem cells.

International journal of hematology

Perdomo-Arciniegas AM,Vernot JP

MHCXCR404 was used in flow cytometry to investigate how mesenchymal stem cells improve hematopoietic stem cell transplantability.

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine receptor; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS

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UniProt ID: (Human) P61073

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Entrez Gene ID: (Human) 7852

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

activation of MAPK activity response to hypoxia neuron migration epithelial cell development dendritic cell chemotaxis apoptotic process inflammatory response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane calcium-mediated signaling telencephalon cell migration entry into host cell regulation of cell migration regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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CXCR4 Monoclonal Antibody (12G5), PE

CXCR4 Antibody (MHCXCR404) in Flow

Product Details

MHCXCR404

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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