Invitrogen

HLA-DR Monoclonal Antibody (TU36), FITC

Product Details

MHLDR01

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

TU36

Immunogen

Human HLA-DR

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374000

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Analyte Specific Reagent

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Product References

Histone deacetylase inhibitors containing a benzamide functional group and a pyridyl cap are preferentially effective human immunodeficiency virus-1 latency-reversing agents in primary resting CD4+ T cells.

The Journal of general virology

Kobayashi Y,Gélinas C,Dougherty JP

MHLDR01 was used in Flow cytometry/Cell sorting to develop a primary cell model of viral latency using primary resting CD4+T cells infected with Vpx-complemented human immunodeficiency virus-1.

Sat Apr 01 00:00:00 EDT 2017

Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin.

Cancer research

Dudek-Perić AM,Ferreira GB,Muchowicz A,Wouters J,Prada N,Martin S,Kiviluoto S,Winiarska M,Boon L,Mathieu C,van den Oord J,Stas M,Gougeon ML,Golab J,Garg AD,Agostinis P

MHLDR01 was used in flow cytometry to study the role of melanoma cell surface-associated calreticulin in melphalan-induced antitumor immunity.

Wed Apr 15 00:00:00 EDT 2015

Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells.

Biochemical pharmacology

Martin S,Dudek-Perić AM,Maes H,Garg AD,Gabrysiak M,Demirsoy S,Swinnen JV,Agostinis P

MHLDR01 was used in flow cytometry to study the role of autophagy in melanoma

Sun Feb 01 00:00:00 EST 2015

ROS-induced autophagy in cancer cells assists in evasion from determinants of immunogenic cell death.

Autophagy

Garg AD,Dudek AM,Ferreira GB,Verfaillie T,Vandenabeele P,Krysko DV,Mathieu C,Agostinis P

MHLDR01 was used in Flow cytometry/Cell sorting to unravel a role for ROS-induced autophagy in weakening functional interaction between dying cancer cells and the immune system.

Sun Sep 01 00:00:00 EDT 2013

Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection.

PloS one

Avanzi S,Leoni V,Rotola A,Alviano F,Solimando L,Lanzoni G,Bonsi L,Di Luca D,Marchionni C,Alvisi G,Ripalti A

MHLDR01 was used in flow cytometry to assess the susceptibility of fetal membranes-derived mesenchymal stromal/stem cells to all members of the human Herpesviridae family.

Mon Apr 14 00:00:00 EDT 2014

Randomized pilot trial of a synbiotic dietary supplement in chronic HIV-1 infection.

BMC complementary and alternative medicine

Schunter M,Chu H,Hayes TL,McConnell D,Crawford SS,Luciw PA,Bengmark S,Asmuth DM,Brown J,Bevins CL,Shacklett BL,Critchfield JW

MHLDR01 was used in Flow cytometry/Cell sorting to study the effect of a synbiotic dietary supplement in the augmentation of probiotic species in the gut during chronic HIV-1 infection.

Fri Jun 29 00:00:00 EDT 2012

Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.

Pediatric research

Boxall S,McCormick J,Beverley P,Strobel S,De Filippi P,Dawes R,Klersy C,Clementi R,De Juli E,Ferster A,Wallace D,Aricò M,Danesino C,Tchilian E

MHLDR01 was used in flow cytometry to study CD45 splice variants in patients with hemophagocytic lymphohistiocytosis.

Mon Mar 01 00:00:00 EST 2004

Chemokine receptor expression in the human ectocervix: implications for infection by the human immunodeficiency virus-type I.

Immunology

Yeaman GR,Asin S,Weldon S,Demian DJ,Collins JE,Gonzalez JL,Wira CR,Fanger MW,Howell AL

MHLDR01 was used in flow cytometry to characterize the frequency of potential targets of HIV infection within the female reproductive tract.

Wed Dec 01 00:00:00 EST 2004

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Journal of virology

Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES

MHLDR01 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.

Tue Jul 01 00:00:00 EDT 2003

Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.

Cytometry. Part B, Clinical cytometry

Braun RK,Foerster M,Grahmann PR,Haefner D,Workalemahu G,Kroegel C

MHLDR01 was used in flow cytometry to test if CD103 has a regulatory role in the microenvironment of the epithelial cell layer.

Tue Jul 01 00:00:00 EDT 2003

Expression of CD41 and c-mpl does not indicate commitment to the megakaryocyte lineage during haemopoietic development.

British journal of haematology

Basch RS,Zhang XM,Dolzhanskiy A,Karpatkin S

MHLDR01 was used in flow cytometry to report that the expression of CD41 and c-mpl were not predictive of commitment to the megakaryocyte lineage.

Tue Jun 01 00:00:00 EDT 1999

CD3 hyporesponsiveness and in vitro apoptosis are features of T cells from both malignant and nonmalignant secondary lymphoid organs.

The Journal of clinical investigation

Agrawal S,Marquet J,Delfau-Larue MH,Copie-Bergman C,Jouault H,Reyes F,Bensussan A,Farcet JP

MHLDR01 was used in Flow cytometry/Cell sorting to compare tumour-infiltrating lymphocytes from B cell non-Hodgkin's lymphomas with T cells from nonmalignant secondary lymphoid organs.

Sun Nov 01 00:00:00 EST 1998

Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1

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Gene Aliases: CD74; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; MLRW; SS1

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UniProt ID: (Human) P04233, (Human) P01903, (Human) Q5UBA2, (Human) P79483, (Human) P13762, (Human) Q30154

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Entrez Gene ID: (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127

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Function(s)

beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding MHC class II receptor activity peptide antigen binding major histocompatibility complex protein scaffold/adaptor protein

Process(es)

activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein complex assembly intracellular protein transport defense response signal transduction cell proliferation immunoglobulin mediated immune response antigen processing and presentation of endogenous antigen antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator T cell selection positive thymic T cell selection negative thymic T cell selection negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II polysaccharide assembly with MHC class II protein complex immune response viral process T cell costimulation T cell receptor signaling pathway cognition interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLA-DR Monoclonal Antibody (TU36), FITC

Product Details

MHLDR01

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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