Invitrogen

Zap-70 Monoclonal Antibody (1E7.2), Alexa Fluor™ 488

13 References

View all (97) Zap-70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Zap-70 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Zap-70 Antibody (MHZAP7020) in Flow

Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human/mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and/or permeabilized using Fix and Perm solution (Product # GAS-003). The profiles represent lymphocyte gate.

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Product Details

MHZAP7020

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E7.2

Immunogen

GST-fusion protein of Human ZAP70, amino acids 282-307

View immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10375316

Product Specific Information

Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Engineering a Dual Small Molecule Gated ZAP70 Switch in T Cells.

ACS synthetic biology

Wong NML,Wong WW

MHZAP7020 was used in Flow cytometry/Cell sorting to develop a dual small molecule-gated ZAP70 signalling switch for the regulation of T cell activity.

Fri Apr 20 00:00:00 EDT 2018

A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70.

The Journal of experimental medicine

Chan AY,Punwani D,Kadlecek TA,Cowan MJ,Olson JL,Mathes EF,Sunderam U,Fu SM,Srinivasan R,Kuriyan J,Brenner SE,Weiss A,Puck JM

MHZAP7020 was used in flow cytometry to identify and characterize human ZAP-70 mutations associated with autoimmune disease

Mon Feb 08 00:00:00 EST 2016

Tipping the Noxa/Mcl-1 balance overcomes ABT-737 resistance in chronic lymphocytic leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Tromp JM,Geest CR,Breij EC,Elias JA,van Laar J,Luijks DM,Kater AP,Beaumont T,van Oers MH,Eldering E

MHZAP7020 was used in Flow Cytometry to demonstrate that the Noxa-Mcl-1 balance determines sensitivity to ABT-737 in CD40-stimulated chronic lymphocytic leukemia cells.

Sun Jan 15 00:00:00 EST 2012

Combined normal donor and CLL: Single tube ZAP-70 analysis.

Cytometry. Part B, Clinical cytometry

Degheidy HA,Venzon DJ,Farooqui MZ,Abbasi F,Arthur DC,Wilson WH,Wiestner A,Stetler-Stevenson MA,Marti GE

MHZAP7020 was used in Flow cytometry/Cell sorting to develop a combined one-tube technology to optimise Zeta-chain-associated protein kinase 70 identification as an independent prognostic marker in chronic lymphocytic leukaemia.

Thu Mar 01 00:00:00 EST 2012

Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregulation of Mcl-1.

Leukemia

Huber S,Oelsner M,Decker T,zum Büschenfelde CM,Wagner M,Lutzny G,Kuhnt T,Schmidt B,Oostendorp RA,Peschel C,Ringshausen I

MHZAP7020 was used in flow cytometry to elucidate the mechanisms of sorafenib-induced cell death.

Sun May 01 00:00:00 EDT 2011

Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia.

Immunology letters

Zucchetto A,Cattarossi I,Nanni P,Zaina E,Prato G,Gilestro M,Marconi D,Bulian P,Rossi FM,Del Vecchio L,Omedè P,Geuna M,Del Poeta G,Gattei V

MHZAP7020 was used in flow cytometry to identify the immunophenotypic signature of chronic lymphocytic leukemia subsets with different prognosis

Sun Jan 30 00:00:00 EST 2011

Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks.

PloS one

Deswal S,Schulze AK,Höfer T,Schamel WW

MHZAP7020 was used in Immunoprecipitation to develop a multi-colour immunoprecipitation technique for studying signal transduction events.

Tue Dec 06 00:00:00 EST 2011

Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells.

Journal of translational medicine

Rossi FM,Del Principe MI,Rossi D,Irno Consalvo M,Luciano F,Zucchetto A,Bulian P,Bomben R,Dal Bo M,Fangazio M,Benedetti D,Degan M,Gaidano G,Del Poeta G,Gattei V

MHZAP7020 was used in Flow cytometry/Cell sorting to assess the prognostic significance of detecting the percentage of ZAP-70 positive cells in chronic lymphocytic leukaemia by three different methods.

Mon Mar 08 00:00:00 EST 2010

High lymphoid enhancer-binding factor-1 expression is associated with disease progression and poor prognosis in chronic lymphocytic leukemia.

Hematology reports

Erdfelder F,Hertweck M,Filipovich A,Uhrmacher S,Kreuzer KA

MHZAP7020 was used in flow cytometry to report that LEF1 expression is associated with poor prognosis and disease progression of chronic lymphocytic leukemia.

Tue Jan 26 00:00:00 EST 2010

ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor.

Leukemia

Zanotti R,Ambrosetti A,Lestani M,Ghia P,Pattaro C,Remo A,Zanetti F,Stella S,Perbellini O,Prato G,Guida G,Caligaris-Cappio F,Menestrina F,Pizzolo G,Chilosi M

MHZAP7020 was used in Flow Cytometry to conclude detection of ZAP-70 on formalin-fixed BM biopsies at diagnosis appears a useful methodological approach to identify patients with poor prognosis in CLL.

Mon Jan 01 00:00:00 EST 2007

Clinical significance of ZAP-70 protein expression in B-cell chronic lymphocytic leukemia.

Blood

Del Principe MI,Del Poeta G,Buccisano F,Maurillo L,Venditti A,Zucchetto A,Marini R,Niscola P,Consalvo MA,Mazzone C,Ottaviani L,Panetta P,Bruno A,Bomben R,Suppo G,Degan M,Gattei V,de Fabritiis P,Cantonetti M,Lo Coco F,Del Principe D,Amadori S

MHZAP7020 was used in Flow cytometry/Cell sorting to suggest that ZAP-70 protein improves the prognostic significance of cytogenetics and may be a better predictor than IgV(H) gene status.

Tue Aug 01 00:00:00 EDT 2006

A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia.

Journal of cellular physiology

Zucchetto A,Bomben R,Dal Bo M,Sonego P,Nanni P,Rupolo M,Bulian P,Dal Maso L,Del Poeta G,Del Principe MI,Degan M,Gattei V

MHZAP7020 was used in flow cytometry describe an immunophenotypic scoring system for B-cell chronic lymphocytic leukemia

Mon May 01 00:00:00 EDT 2006

ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.

Cytometry. Part B, Clinical cytometry

Best OG,Ibbotson RE,Parker AE,Davis ZA,Orchard JA,Oscier DG

MHZAP7020 was used in Flow cytometry/Cell sorting to support a rapid method for ZAP-70 measurement using commercially available fixation/permeabilization reagents, a conjugated antibody, and a ratiometric method of analysis that minimizes subjective interpretation of the results.

Sat Jul 15 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

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Gene Aliases: ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70

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UniProt ID: (Human) P43403, (Mouse) P43404

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Entrez Gene ID: (Human) 7535, (Mouse) 22637

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Function(s)

phosphotyrosine binding protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response protein phosphorylation inflammatory response immune response transmembrane receptor protein tyrosine kinase signaling pathway peptidyl-tyrosine phosphorylation T cell differentiation positive regulation of cell adhesion mediated by integrin intracellular signal transduction peptidyl-tyrosine autophosphorylation T cell activation B cell activation positive regulation of mast cell degranulation beta selection positive thymic T cell selection negative thymic T cell selection innate immune response positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway T cell aggregation T cell migration regulation of platelet aggregation immune system process phosphorylation cell migration cell differentiation thymic T cell selection protein autophosphorylation

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Zap-70 Monoclonal Antibody (1E7.2), Alexa Fluor™ 488

Zap-70 Antibody (MHZAP7020) in Flow

Product Details

MHZAP7020

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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