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MICA/B Antibody (PA5-109323) in ICC/IF
Immunofluorescent analysis of MICA + MICB in A431 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with MICA + MICB polyclonal antibody (Product # PA5-109323) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Product Details
PA5-109323
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human MICA + MICB aa 182-325
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854734
Product Specific Information
Positive Control: U937, Mouse bone marrow, A431, rat skin tissue, human lung cancer tissue, human colon tissue, human breast carcinoma tissue, mouse stomach tissue.
Target Information
MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MIC-A; MIC-B; MICA; MICB; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A
Gene Aliases: MIC-A; MICA; MICB; PERB11.1; PERB11.2
UniProt ID: (Human) Q9TQA4, (Human) Q29980
Entrez Gene ID: (Human) 100507436, (Human) 4277
T cell mediated cytotoxicity
immune response to tumor cell
cellular response to DNA damage stimulus
response to heat
cytolysis
antigen processing and presentation
negative regulation of natural killer cell activation
natural killer cell mediated cytotoxicity
defense response to bacterium
negative regulation of natural killer cell mediated cytotoxicity
gamma-delta T cell activation
regulation of immune response
defense response to virus
immune response-activating cell surface receptor signaling pathway
response to oxidative stress
viral process
response to retinoic acid
negative regulation of defense response to virus by host
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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